SOLUBLE ADENYLYL CYCLASE ACTIVITY IN MALE GERM CELLS

描述：(改编自申请者的摘要)Camp，一个几乎普遍的 信号分子，是由三磷酸腺苷合成的一大类腺苷 环酶(AC)。在哺乳动物中，AC有两种类型。一个表单跨越 膜是由一个基因家族编码的，该基因家族由九个相关的 跨膜急性冠脉综合征(TMAC)。这些受G蛋白和其他第二种蛋白的调节 信使。私家侦探最近发现了第二种哺乳动物的AC， 可溶性AC(SAC)，对已知的tmAC和DO调节剂不敏感 不具有膜跨结构域。麦迪和SAC似乎扮演着相反的角色 对细胞增殖的影响，并由不同的 药理制剂。小苏打的活性受小苏打离子的直接调节。 由于SAC优先在雄性生殖细胞中表达，并且是已知的唯一 研究人员提出，碳酸氢盐刺激哺乳动物的腺酰环化酶 它是在精子运动方面发挥作用的一个很好的候选者， 电容化和顶体反应。拟议的研究将确定 SAC的调节和酶学特性及其在药理和应用中的应用 用遗传学方法测试SAC在生殖功能中的作用。长期 计划包括将SAC发展为潜在的药理靶点 影响生育的治疗方法。

DESCRIPTION: (Adapted from the applicant's abstract) cAMP, a nearly universal signaling molecule, is synthesized from ATP by a broad family of adenylyl cyclases (AC). In mammals, there are two types of AC. One form spans the membrane and is encoded by a gene family consisting of nine related transmembrane ACs (tmAC). These are regulated by G proteins and other second messengers. The P.I. has recently identified a second type of mammalian AC, soluble AC (sAC) that is insensitive to the known modulators of tmACs and does not possess membrane spanning domains. tmAC and sAC appear to mediate opposite effects on cellular proliferation and are differentially modulated by pharmacological agents. sAC activity is directly regulated by bicarbonate ions. Since sAC is preferentially expressed in male germ cells and is the only known bicarbonate stimulated adenylyl cyclase in mammals, the investigators propose that it is an excellent candidate to play roles in aspects of sperm motility, capacitiation and the acrosome reaction. The proposed studies will determine the regulatory and enzymological profile of sAC and use pharmacological and genetic approaches to test the role of sAC in reproductive function. Long term plans include developing sAC as a potential target for pharmacological therapeutics affecting fertility.