BIOPHYSICAL STUDIES OF CYTOSKELETAL FUNCTION

细胞骨架功能的生物物理学研究

• 批准号: 6385698
• 负责人: Elliot L. Elson
• 金额: $ 28.98万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6385698
• 关键词: actins; biological signal transduction; biophysics; cell adhesion; cell cell interaction; cell motility; cytoskeleton; dystrophin; extracellular matrix; fibroblasts; integrins; mechanical pressure; microtubules; myosins; phospholipase C; tissue /cell culture; transfection; viscosity

DESCRIPTION: The goal of the proposed experiments is to address two broad questions. How do non-muscle tissue cells regulate the contractile forces they exert on each other and on the extracellular matrices in which they are embedded? To what extent and how can these cells sense external forces exerted on them and respond biochemically and mechanically. Six goals are proposed : (1) to determine the contribution of different signaling pathways to the activation of myosin and the exertion of contractile force; (2) to determine how myosin activation results from disruption of the microtubule network; (3) to determine the pathway by which phospholipase C delta1 contributes to generation of contractile force in fibroblasts; (4) to determine the roles of selected actin filament capping proteins in controlling the stiffness of the actin cytoskeleton; (5) to determine how adhesion to the ECM contributes to development of tissues and the exertion of force; and (6) to determine whether the exertion of force on cells via integrin-ligand interactions has important regulatory functions.

描述：拟议实验的目标是解决两个广泛的 问题. 非肌肉组织细胞如何调节收缩力 它们相互作用并作用于它们所处的细胞外基质， 嵌入式？ 这些细胞能在多大程度上以及如何感知外力 施加在它们身上并做出生物化学和机械反应。 六个目标是 建议：（1）确定不同信号通路的贡献 肌球蛋白的激活和收缩力的发挥;（2） 确定肌球蛋白激活如何导致微管的破坏 网络;（3）确定磷脂酶C δ 1 有助于在成纤维细胞中产生收缩力;（4） 确定选定的肌动蛋白丝帽蛋白在 控制肌动蛋白细胞骨架的刚度;（5）确定如何 与ECM的粘附有助于组织的发育， （6）确定是否通过对细胞施加力 整联蛋白-配体相互作用具有重要的调节功能。

项目成果

Correlation of myosin light chain phosphorylation with isometric contraction of fibroblasts.

• 发表时间: 1993-11
• 期刊: The Journal of biological chemistry
• 作者: M. Kolodney;Elliot L. Elson

Cell poking: quantitative analysis of indentation of thick viscoelastic layers.

• DOI: 10.1016/s0006-3495(89)82867-x
• 发表时间: 1989-04
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: M. Duszyk;B. Schwab;G. Zahalak;H. Qian;E. Elson

Kinetics and mechanism of the folding of cytochrome c.

细胞色素c折叠的动力学和机制。

• DOI: 10.1021/bi00137a006
• 发表时间: 1992
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Pryse,KM;Bruckman,TG;Maxfield,BW;Elson,EL
• 通讯作者: Elson,EL

Phenotypic screening for pharmaceuticals using tissue constructs.

使用组织构建体进行药物表型筛选。

• DOI: 10.2174/1389201043376940
• 发表时间: 2004
• 期刊: Current pharmaceutical biotechnology
• 影响因子: 2.8
• 作者: Wakatsuki,T;Fee,JA;Elson,EL
• 通讯作者: Elson,EL

One-dimensional viscoelastic behavior of fibroblast populated collagen matrices.

成纤维细胞填充的胶原基质的一维粘弹性行为。

• DOI: 10.1115/1.1614818
• 发表时间: 2003
• 期刊: Journal of biomechanical engineering
• 作者: Wagenseil,JessicaE;Wakatsuki,Tetsuro;Okamoto,RuthJ;Zahalak,GeorgeI;Elson,ElliotL
• 通讯作者: Elson,ElliotL