OXIDATION OF PYROLITIC CARBON: PROTEIN & CELL ADHESION

热解碳的氧化：蛋白质

• 批准号: 6345051
• 负责人: GRACE PICCIOLO
• 金额: $ 1.23万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345051
• 关键词: bioengineering /biomedical engineering; biomedical resource; health care; human tissue; injury; skin

This study aims to determine whether human monocytes/macrophages cultured on pyrolytic carbon (PYC) can oxidize the surface. Oxidation of the pyrolitic carbon is achieved by a number of methods, and surface analytical methods are employed to compare the surfaces so obtained to those observed after cell adhesion studies have been performed. SIMS was employed to characterize the prepared surface as well as to determine how successful various cleaning strategies are in removing adherent proteins and cells, which is necessary for subsequent sample analysis.

本研究旨在确定人单核细胞/巨噬细胞是否 在热解碳（PYC）上培养可以氧化表面。 氧化 通过多种方法获得热解碳， 采用表面分析方法来比较表面， 在细胞粘附研究后观察到的那些， 执行。 采用西姆斯表征制备的表面， 以及确定各种清洁策略在 去除粘附的蛋白质和细胞， 随后的样品分析。