OXIDATION OF PYROLITIC CARBON: PROTEIN & CELL ADHESION

本研究旨在确定人类单核/巨噬细胞 在热解碳(PYC)上培养可以氧化表面。氧化 通过多种方法获得热解碳，以及 表面分析方法被用来比较表面因此 在细胞黏附研究后观察到的那些 已执行。用SIMS对所制备的表面进行了表征 以及确定各种清洁策略在 去除黏附的蛋白质和细胞，这是 随后的样本分析。

This study aims to determine whether human monocytes/macrophages cultured on pyrolytic carbon (PYC) can oxidize the surface. Oxidation of the pyrolitic carbon is achieved by a number of methods, and surface analytical methods are employed to compare the surfaces so obtained to those observed after cell adhesion studies have been performed. SIMS was employed to characterize the prepared surface as well as to determine how successful various cleaning strategies are in removing adherent proteins and cells, which is necessary for subsequent sample analysis.