ARTIFACTS IN GRADIENT ENHANCED HSQC

梯度增强 HSQC 中的伪影

• 批准号: 6355157
• 负责人: CHRISTOPHER TURNER
• 金额: $ 0.64万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6355157
• 关键词: biological products; biomedical resource; proteins

The Gradient Enhanced Heteronuclear Single Quantum Correlation (GEHSQC) experiment is widely used, especially as a building block in multi-dimensional NMR, for instance in 3D NOESY HSQC experiments. However, close inspection of GEHSQC spectra reveals a large number of small artifacts. In situations of high dynamic range, these artifacts can interfere with spectral interpretation, since the artifacts associated with intense signals can obscure weak correlations of interest. We have sought to explain the origin of these artifacts and find a method to suppress them. They turn out to be caused by the evolution of proton scalar coupling during the back-transfer portion of the pulse sequence via a relayed coherence transfer mechanism. The artifacts appear as cross-peaks involving remote protons. Product operator analysis of artifact formation and experimental results demonstrate that the magnitude of these artifacts can exceed 10% of the main peak. Therefore, these artifacts represent a significant loss in sensitivity.

梯度增强异核单量子相关性 （GEHSQC）实验被广泛使用，特别是作为构建块 多维 NMR，例如 3D NOESY HSQC 实验。 然而，对 GEHSQC 光谱的仔细检查揭示了大量 小文物。 在高动态范围的情况下，这些伪影 可能会干扰光谱解释，因为伪影 与强信号相关可以掩盖弱相关性 兴趣。 我们试图解释这些文物的起源 找到一种方法来压制它们。 事实证明它们是由 反向传输部分期间质子标量耦合的演变 通过中继相干传输机制的脉冲序列。 这 伪影表现为涉及远程质子的交叉峰。 产品 伪影形成和实验结果的操作员分析 证明这些伪影的大小可以超过 10% 主峰。 因此，这些文物代表了重要的 敏感性丧失。