ENHANCED ELECTORHEOLOGICAL FLUIDS USING HYERPTHERMOPHILIC BIOTECHNOLOGY

使用嗜热生物技术增强电流变液体

• 批准号: 6307856
• 负责人: KENNETH H NEALSON
• 金额: $ 1.13万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6307856
• 关键词: bioengineering /biomedical engineering; biomedical resource; biotechnology; congenital disorders; plants

Microorganism Shewanella putrefaciens can grow with a variety of terminal acceptors, including oxygen, nitrate, nitrite, TMAO, Fe(3+), Mn(4+), etc. Such diversity admits that synthesis of terminal reductases could be induced by applied acceptors. To study electron transport chain of S. putrefaciens, we used low temperature EPR spectroscopy at different frequencies. It has been shown that, depending on the accessible electron acceptor studied, the microorganism developed different electron carriers. The growth with Fe(3) as the terminal electron acceptor induced the appearance of multiheme cytochromes type 2 low (ca 10 kD) and high (70 kD) molecular weight. Both cytochromes have four heme groups per protein molecule. We use low temperature EPR spectroscopy to study interactions between heme groups in the same protein molecule.

微生物腐坏希瓦氏菌可以生长在多种