BIOLOGICAL CONSEQUENCES OF SLOW NEUTRONS

一个高LET染色体生物标志物的鉴定 辐射将有助于识别受辐射的个人。 不仅对家庭和职业中的氡可能负有责任 对于特定的健康影响，也对裂变的中子。研究 已经提出，高LET辐射产生低比率[F] 染色体间到染色体内臂间交换像差与 低LET辐射。用多色荧光原位检测技术 杂交[鱼]画我们分析了频率间和 染色体内交换。正常人成纤维细胞处于G1期和 G0人淋巴细胞受2-8Gy0.6Gy/hr137Cs-γ射线照射 430千电子伏中子0.2-0.8Gy射线，或0.2Gy65千电子伏特 中子。研究发现，F-比率具有剂量依赖性和混乱性 通过高LET后观察到的复杂交换的高比例 辐射。观察到较高频率的复杂交流后 中子辐射[22-30%]，而LDR伽马射线[8-22%]。这个 在淋巴细胞中发现复杂交换的频率更高 比成纤维细胞，尤其是在高LET辐射后。这些 研究鼓励考虑使用这种方法来分析 潜在的可存活的染色体变化。

The identification of a chromosomal biomarker for high LET radiation would facilitate the identification of individuals exposed not only to domestic and occupational radon which may be responsible for specific health effects, but also to fission neutrons. Studies have suggested that high LET radiation produces a low ratio [F] of inter- to intrachromosome interarm exchange aberrations compared with low LET radiation. Using multicolor fluorescence in situ hybridization [FISH] painting we analyzed the frequency of inter- and intrachromosome exchanges.Normal human fibroblasts in the G1 phase and G0 human lymphocytes were exposed to 2-8 Gy of 0.6 Gy /hr 137 Cs gamma -rays , 0.2-0.8 Gy of 430 keV neutrons , or 0.2-0.8 Gy 65 keV neutrons. The F-ratio was found to be dose dependent and confounded by the high proportion of complex exchanges observed after high LET radiation. A higher frequency of complex exchanges was observed after neutron radiation [22-30%] compared with LDR gamma -rays [8-22%]. The frequency of complex exchanges was found to be higher in lymphocytes than fibroblasts , particularly after high LET radiation. These studies encourage consideration of this approach for the analysis of latent viable chromosomal changes.