ANALYSIS OF RNA POLYMERASE II TRANSCRIPTION COMPLEXES BY TANDEM MASS SPECT

通过串联质谱分析 RNA 聚合酶 II 转录复合物

• 批准号: 6348289
• 负责人: DAVID SCHIELTZ
• 金额: $ 0.33万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6348289
• 关键词: bioengineering /biomedical engineering; biomedical resource; genome; microorganism; plants; proteins; technology /technique

A screen of the yeast activation domain array was carried out using wild-type Cdc28 and a kinase-dead mutant, Cdc28K30R, as baits. The DNA-binding domain fusion constructs were constructed in the Futcher laboratory and sent to the Resource Center. The first screen of the wild-type Cdc28 bait on -histidine media + 2mM 3-aminotriazole showed a somewhat high His+ background, but 13 positives were clearly visible above background, including three proteins known to interact with Cdc28.

对酵母激活结构域阵列进行了筛选 以野生型CDC28和激酶死亡突变体CDC28K30R为诱饵。 DNA结合区融合构建体构建于 福彻实验室，并被送到资源中心。第一个屏幕 组氨酸+2 mM 3-氨基三氮唑对野生型Cdc28诱饵的影响 显示出他的背景有点高，但13个阳性明显 在背景上方可见，包括三种已知相互作用的蛋白质 用Cdc28。