USE OF MULTILAYER MONOCHROMATORS FOR MACROMOLECULAR CRYSTALLOGRAPHY

多层单色仪在高分子晶体学中的应用

• 批准号: 6346387
• 负责人: MALCOLM S CAPEL
• 金额: $ 16.31万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6346387
• 关键词: bioimaging /biomedical imaging; biological products; biomedical equipment development; biomedical resource; proteins; radiography; radiology; structural biology; technology /technique

Crystallographic studies of weakly-diffracting biological samples using bending-magnet beamlines at the NSLS frequently are limited by the low intensity of the focussed, monochromatic X-ray beam striking the sample. For example, direct comparison of beamlines X4A, X8C X I 2B, and X I 2C (bending magnets) with beamline X25 (wiggler) demonstrated that the bending-magnet beamlines do not offer sufficient flux for studies of some of the most interesting and difficult crystallographic problems - large macromolecular complexes - at their highest possible resolution limits. With the growing number of protein crystallography laboratories in both academe and industry, wiggler beamlines suitable for routine x-ray diffraction data collection from the most weakly diffracting specimens are now heavily over- subscribed. Acknowledging the difficulty in simply building more wiggler beamlines, one would like to find an alternative means to provide dramatically more x-ray flux at the NSLS so that one ca n approach these difficult problems.

弱衍射型生物样品的结晶学研究 在NSLS频繁使用弯曲磁铁光束线受到以下限制 聚焦单色X射线束击打的低强度 样本。例如，直接比较光束线X4A、X8C、XI 2B和X I 2C(弯曲磁铁)，带光束线X25(摇摆器) 证明了弯曲磁铁光束线不能提供足够的 通量研究的一些最有趣和最困难的 结晶学问题--大分子络合物-- 可能的最高分辨率限制。随着越来越多的 学术界和工业界的蛋白质结晶学实验室 适用于常规X射线衍射数据的摆动光束线 从最弱的衍射样品中收集的样品现在很多 超额认购。认识到简单地建造 更多的摆动光束线，人们想要找到一种替代方法来 在NSLS提供显著更多的X射线通量，因此 解决这些难题。