USE OF MULTILAYER MONOCHROMATORS FOR MACROMOLECULAR CRYSTALLOGRAPHY

多层单色仪在高分子晶体学中的应用

• 批准号: 6310148
• 负责人: MALCOLM S CAPEL
• 金额: $ 16.31万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6310148
• 关键词: bioimaging /biomedical imaging; biological products; biomedical equipment development; biomedical resource; proteins; radiography; radiology; structural biology; technology /technique

Crystallographic studies of weakly-diffracting biological samples using bending-magnet beamlines at the NSLS frequently are limited by the low intensity of the focussed, monochromatic X-ray beam striking the sample. For example, direct comparison of beamlines X4A, X8C X I 2B, and X I 2C (bending magnets) with beamline X25 (wiggler) demonstrated that the bending-magnet beamlines do not offer sufficient flux for studies of some of the most interesting and difficult crystallographic problems - large macromolecular complexes - at their highest possible resolution limits. With the growing number of protein crystallography laboratories in both academe and industry, wiggler beamlines suitable for routine x-ray diffraction data collection from the most weakly diffracting specimens are now heavily over- subscribed. Acknowledging the difficulty in simply building more wiggler beamlines, one would like to find an alternative means to provide dramatically more x-ray flux at the NSLS so that one ca n approach these difficult problems.

弱衍射生物样品的晶体学研究 在国家空间激光发射系统中使用弯曲磁铁束线，常常受到以下因素的限制 聚焦的单色X射线束的低强度撞击 示例. 例如，光束线X4 A、X8 C X 1 2B和X I 2C（弯曲磁体），带束线X25（摆动器） 表明弯曲磁体束线不能提供足够的 一些最有趣和最困难的研究 晶体学问题-大分子复合物-在其 最高分辨率极限。 随着越来越多的 蛋白质晶体学实验室在工业和工业， 适用于常规X射线衍射数据的摆动器光束线 从最弱的衍射标本收集，现在是沉重的 超额认购。 承认简单地建立 更多的摆动器光束线，人们希望找到一种替代手段， 在NSLS上提供更大的X射线通量， 解决这些难题。