TB LAM AND AG--STRUCTURES CORRELATED WITH BIOLOGY
TB LAM 和 AG——与生物学相关的结构
基本信息
- 批准号:6373436
- 负责人:
- 金额:$ 18.65万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1996
- 资助国家:美国
- 起止时间:1996-06-01 至 2003-06-30
- 项目状态:已结题
- 来源:
- 关键词:Mycobacterium tuberculosis SDS polyacrylamide gel electrophoresis T lymphocyte bacterial antigens carbohydrate receptor carbohydrate structure cell wall cellular immunity chemical structure function drug resistance galactans macrophage mannans mass spectrometry nuclear magnetic resonance spectroscopy receptor sensitivity surface antigens
项目摘要
DESCRIPTION (Adapted from the Applicant's Abstract): Important physiological
and immunopathogenic features of mycobacteria including slow growth, acid
fastness, resistance to drug permeation, and immunological persistence have
been attributed to the presence of lipoarabinomannan (LAM) and arabinogalactan
(AG) in the cell wall. This competitive renewal of Al 37139 is built on success
in the initial funding period of determining structural characteristics of
truncated LAM from laboratory induced ethambutol (Emb) resistant M. smegmatis
and LAM and AG from clinical isolates of M. tuberculosis resistant to Emb. To
take the next step in fully understanding the cell surface of M. tuberculosis
and its manifestation in bacterial physiology and pathology, it is proposed to
determine the entire structures of LAM and AG, to characterize LAM and AG in a
panel of Emb resistant M. tuberculosis isolates, and, in collaboration, to
determine and precise structures of LAM needed for CD1 T-cell and macrophage
mannose receptor recognition. Although some structural features of LAM and AG
have been delineated, key issues, including the structure of the arabinan
regions of both polymers, and the site of the arabinan attachments to the
hexose backbones of each polymer remain unknown. To obtain the complete primary
structure of these two 100 glycosyl residues glycans, novel cell wall degrading
enzymes will be used to prepare fragments whose structures will be determined
by state-of-the-art nuclear magnetic resonance (NMR) and mass spectrometry (MS)
analysis. The enzymes include seven novel arabinanases and galactofuranases
secreted by a Cellulomonas species and an endo alpha-1,6 mannosidase from
Bacillus circulans. NMR will focus on the analysis of fully 1 3C labeled
polysaccharides and polysaccharide fragments by 2-D and 3-D techniques. Mass
Spectrometry will feature a novel Q-TOF-tandem electrospray mass spectrometer
to obtain sequence information. Techniques based on SDS-PAGE, endo-arabinanase
cleavage, 1-D-NMR, and MS will be used to efficiently characterize LAM and AG
from our panel of Emb resistant M. tuberculosis. The structural studies will
directly aid the immunological studies by providing a wealth of LAM related
molecules needed to decipher the structural characteristics necessary for
recognition by T-cells and macrophages.
描述(改编自申请人摘要):重要的生理
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DELPHI CHATTERJEE其他文献
DELPHI CHATTERJEE的其他文献
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{{ truncateString('DELPHI CHATTERJEE', 18)}}的其他基金
Validation of urine/serum LAM in HIV/nonHIV TB suspects and POC Test Development
HIV/非 HIV 结核病疑似者的尿液/血清 LAM 验证和 POC 测试开发
- 批准号:
10179309 - 财政年份:2018
- 资助金额:
$ 18.65万 - 项目类别:
Validation of urine/serum LAM in HIV/nonHIV TB suspects and POC Test Development
HIV/非 HIV 结核病疑似者的尿液/血清 LAM 验证和 POC 测试开发
- 批准号:
9925722 - 财政年份:2018
- 资助金额:
$ 18.65万 - 项目类别:
Biosynthesis and transbilayer flipping of mycobacterial PIM glycolipids
分枝杆菌 PIM 糖脂的生物合成和跨双层翻转
- 批准号:
7511622 - 财政年份:2008
- 资助金额:
$ 18.65万 - 项目类别:
Biosynthesis and transbilayer flipping of mycobacterial PIM glycolipids
分枝杆菌 PIM 糖脂的生物合成和跨双层翻转
- 批准号:
7632133 - 财政年份:2008
- 资助金额:
$ 18.65万 - 项目类别:
Anti MDR-TB MICs and Cytotoxicity of New Compounds
新化合物的抗耐多药结核病 MIC 和细胞毒性
- 批准号:
6735405 - 财政年份:2003
- 资助金额:
$ 18.65万 - 项目类别:
M AVIUM WALL STRUCTURES FROM AIDS PATIENTS
来自艾滋病患者的 M AVIUM 墙结构
- 批准号:
2751059 - 财政年份:1997
- 资助金额:
$ 18.65万 - 项目类别:
M AVIUM WALL STRUCTURES FROM AIDS PATIENTS
来自艾滋病患者的 M AVIUM 墙结构
- 批准号:
2545040 - 财政年份:1997
- 资助金额:
$ 18.65万 - 项目类别:
M AVIUM WALL STRUCTURES FROM AIDS PATIENTS
来自艾滋病患者的 M AVIUM 墙结构
- 批准号:
6044167 - 财政年份:1997
- 资助金额:
$ 18.65万 - 项目类别:
Structure of LAM in Relation to Biology and Biosynthesis
LAM 结构与生物学和生物合成的关系
- 批准号:
6544799 - 财政年份:1996
- 资助金额:
$ 18.65万 - 项目类别:
STRUCTURE OF LAM IN RELATION TO BIOLOGY AND BIOSYNTHESIS
LAM 结构与生物学和生物合成的关系
- 批准号:
2672427 - 财政年份:1996
- 资助金额:
$ 18.65万 - 项目类别:














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