STUDIES OF THE COCHLEAR NUCLEUS GRANULE CELL DOMAIN

耳蜗核颗粒细胞域的研究

• 批准号: 6379540
• 负责人: DAVID K. RYUGO
• 金额: $ 30.74万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2005-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379540
• 关键词: auditory cortex; auditory discrimination; auditory nuclei; auditory stimulus; axon; brain electrical activity; brain mapping; cats; cerebellum; electron microscopy; granule cell; immunocytochemistry; injection /infusion; laboratory rat; light microscopy; mesencephalon; molecular site; neural information processing; neuronal transport; neurons; pons; radiotracer; sensorimotor system; sound frequency; synaptic vesicles

DESCRIPTION: (Adapted from the Investigator's Abstract) Our long-term goal is to understand neuronal mechanisms that underlie hearing. All acoustic information from the environment enters the brain via the auditory nerve and synpases in the cochlear nucleus. The cochlear nucleus in turn gives rise to the ascending pathways. The perception of sound is normally attributed to processing by these ascending pathways, but data are accumulating that stimulus context also plays a major role. That is, the meaning of a sound depends not only on spectral and temporal characteristics, but also on the past history and behavioral state of the animal. This contextual information could be provided by descending auditory pathways and neural systems not traditionally considered "auditory." In this application, we plan to study the interface between these ascending and descending systems. We propose that the granule cell domain of the cochlear nucleus represents a key site for the integration of multimodal influences. Preliminary data reveal that, in addition to descending auditory projections, there are somatosensory, vestibular and motor inputs to the granular cell domain. For the present application, we will apply anterograde and retrograde tract tracing methods to identify these neurons and their circuits, immunocytochemical staining procedures to reveal the chemistry of the different neurons and their terminals, and electrophysiological recording techniques to characterize the response properties of neurons projecting to this region. The data from this research will provide new knowledge on the synpatic organization of the highly integrative structure in the auditory system, and yield insights into how diverse neural systems shape the coding process for hearing.

描述：（改编自研究者摘要）我们的长期目标是 了解听力背后的神经机制。全声学 来自环境的信息通过听觉神经进入大脑 耳蜗核中的突触。耳蜗核反过来产生 上升路径。对声音的感知通常归因于 通过这些上升路径进行处理，但数据正在积累该刺激 背景也起着重要作用。也就是说，声音的含义并不取决于 不仅涉及光谱和时间特征，还涉及过去的历史和 动物的行为状态。可以提供此上下文信息 通过传统上未考虑的下行听觉通路和神经系统 “听觉。”在这个应用程序中，我们计划研究这些之间的接口 上升和下降系统。 我们认为耳蜗核的颗粒细胞域代表 多式联运影响整合的关键场所。初步数据揭晓 除了下降的听觉投射之外，还有体感， 前庭和运动输入到颗粒细胞域。就目前而言 应用程序中，我们将应用顺行和逆行束追踪方法 识别这些神经元及其回路，免疫细胞化学染色 揭示不同神经元及其化学性质的程序 终端和电生理记录技术来表征 投射到该区域的神经元的反应特性。数据来自于此 研究将提供关于高度神经元突触组织的新知识 听觉系统中的整合结构，并深入了解如何 不同的神经系统塑造听力的编码过程。