CANDIDATE GENES FOR DG1 AND ADAI

DG1 和 ADAI 的候选基因

• 批准号: 6379657
• 负责人: Mary MacDougall
• 金额: $ 21.49万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379657
• 关键词: ameloblasts; artificial chromosomes; autosomal dominant trait; bone sialoprotein; clinical research; dental disorder; dentin; dentinogenesis; extracellular matrix proteins; family genetics; fluorescent in situ hybridization; gene mutation; genetic disorder; genetic library; genetic markers; genotype; human genetic material tag; human subject; linkage mapping; molecular pathology; northern blottings; phosphoproteins; polymerase chain reaction; single strand conformation polymorphism; southern blotting

Relatively little in known about the molecular etiology of the human genetic diseases of enamel and dentin formation: amelogenesis imperfecta (AI), dentinogenesis imperfecta (DGI), and dentin dysplasia (DD). Linkage studies for autosomal dominant (AD) forms of AI, approximately 85% of all cases, have mapped only one type, local hypoplastic AI (AIH2), to human chromosome 4q21 within a 4 Mb region containing the genes for ameloblastin and enamelin. This same general region of 4q21 has been shown to contain the overlapping critical gene loci for three dentin diseases, DGI type II (DGI-II), DGI type III (DGI-III), and DD type II (DD-II). This overlapping region contains a dentin/bone "gene cluster", including osteopontin, bone sialoprotein, dentin matrix protein I, and dentin sialophosphoprotein. Mutational analysis studies have yet to establish the genes causing these diseases. The goal of this R01 continuation renewal is the delineation of the primary genetic defects that are responsible for the diseases DGI-II, DGI-III, DD- II, and the AD forms of AI. We will expand our efforts to include DD type II and other AD forms of AI through newly ascertained informative families. This study is based on the hypothesis that specific mutations within single dentin and enamel matrix proteins/proteinases have a causative role in the pathogenesis of DGI, DD and the AD forms of AI. To test this hypothesis we will ascertain informative families with these dental genetic diseases, establish or exclude linkage to the DGI/DD or AIH2 Critical disease loci on 4q21, and perform mutational analysis of potential candidate genes. Refined linkage studies will be performed on these disease loci through more extensive marker genotyping of additional families. ADAI families excluded from 4q2i, will be tested for linkage to other candidate enamel gene loci on chromosomes 1, 11, and 19, and the critical disease loci mapped. If ADAI families are excluded from linkage to all potential gene loci, a genome-wide search will be implemented. Finally, through positional cloning approaches identify potential new candidate tooth genes on 4q21 using constructed DGI/DD and AIH2 contigs. This information will contribute to our understanding of the molecular bases of these diseases, provide better diagnosis tools, increase our knowledge related to the function(s) of tooth proteins, and assisting in the design of treatment therapies.

人类遗传性牙釉质和牙本质形成疾病的分子病因学研究相对较少，包括牙釉质发育异常（AI）、牙本质发育异常（DGI）和牙本质发育不良（DD）。约85%的常染色体显性（AD）形式的AI的连锁研究，只有一种类型，局部发育不良AI（AIH 2），映射到人类染色体4 q21的4 Mb区域内含有成釉蛋白和釉蛋白的基因。4 q21的这个相同的一般区域已被证明包含三种牙本质疾病的重叠关键基因位点，DGI II型（DGI-II），DGI III型（DGI-III）和DD II型（DD-II）。该重叠区域包含牙本质/骨“基因簇”，包括骨桥蛋白、骨唾液蛋白、牙本质基质蛋白I和牙本质唾液磷蛋白。突变分析研究尚未确定导致这些疾病的基因。该R 01延续更新的目标是描述导致疾病DGI-II、DGI-III、DD- II和AD形式AI的主要遗传缺陷。我们将通过新确定的信息家族扩大我们的努力，包括DD II型和其他AD形式的AI。本研究基于以下假设：单个牙本质和釉质基质蛋白/蛋白酶内的特定突变在DGI、DD和AD形式的AI的发病机制中具有致病作用。为了验证这一假设，我们将确定这些牙齿遗传性疾病的信息家族，建立或排除4 q21上DGI/DD或AIH 2关键疾病位点的连锁，并对潜在的候选基因进行突变分析。将通过对其他家族进行更广泛的标记基因分型，对这些疾病位点进行精细的连锁研究。从4 q2 i中排除的ADAI家族将被检测与染色体1、11和19上的其他候选釉质基因位点的连锁，并绘制关键疾病位点。如果ADAI家族被排除在与所有潜在基因位点的连锁之外，则将实施全基因组搜索。最后，通过位置克隆方法，使用构建的DGI/DD和AIH 2重叠群在4 q21上鉴定潜在的新候选牙齿基因。这些信息将有助于我们了解这些疾病的分子基础，提供更好的诊断工具，增加我们对牙齿蛋白质功能的了解，并有助于设计治疗方法。