FORMATION OF THE DROSOPHILA SALIVARY GLAND
果蝇唾液腺的形成
基本信息
- 批准号:6223586
- 负责人:
- 金额:$ 26.85万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-02-01 至 2006-01-31
- 项目状态:已结题
- 来源:
- 关键词:Drosophilidae arthropod genetics biological signal transduction cell migration developmental genetics embryo /fetus tissue /cell culture gene expression gene mutation gene targeting genetic regulation histogenesis molecular cloning mutant protein protein interaction protein structure function salivary glands transcription factor
项目摘要
DESCRIPTION (Adapted from the Investigator's Abstract): The long-term goal of
this research is to understand in molecular detail how cells choose to form a
particular organ and how they carry out this decision. The Principal
Investigator proposes to address these questions by a systematic analysis of
formation of a simple tissue, the salivary glands, in a genetically-tractable
organism, the fruit fly, Drosophila melanogaster. In the past several years,
the Principal Investigator has identified the factors that control where
salivary glands will form and the number of cells committed to a salivary gland
fate. In the next few years, the proposed studies will provide information on
how these factors work in combination to control expression of salivary gland
genes. The Principal Investigator also has perhaps the largest single
collection of genes expressed in a specific tissue. Her goal is to pinpoint and
characterize the genes that function very early in salivary gland development,
particularly genes that coordinate the major morphogenetic movements required
to internalize the salivary glands and to form functional epithelial tubes. To
achieve these goals, four specific aims are proposed. In Aim 1, studies are
proposed to test if activation of three early salivary gland genes by the
transcription factors SCR, EXD and HTH is direct and, if so, will learn how
TSH, ABD-B and DPP-signaling block activation. In Aim 2, studies are proposed
to address the role of two early transcription factors, FKH and HKB, in
salivary gland internalization. Fkh mutants fail to complete internalization of
the salivary gland and show extensive salivary cell death. Hkb mutants initiate
internalization at the wrong place and form abnormally shaped salivary glands.
In Aim 3, studies are proposed to address the role of two genes, rib and
D-SemaII, in positioning the salivary gland. Rib mutants have defects in
directed migration of salivary gland cells and in D-SemaII mutants the distal
portion of the salivary gland is abnormally positioned. In Aim 4, studies are
proposed to find new genes required for salivary gland morphogenesis. These new
genes will be identified both from the Principal Investigator's collection of
genes known to be expressed in the developing salivary gland and from an EMS
saturation screen. The proposed studies will provide a working blueprint for
the early events of organ formation in all higher organisms.
描述(改编自《调查员摘要》):的长期目标
这项研究是为了从分子上详细了解细胞如何选择形成
特别机构以及他们如何执行这一决定。《校长》
研究人员建议通过系统分析这些问题来解决这些问题
形成一种简单的组织,唾液腺,在遗传上容易驯服
有机体,果蝇,黑腹果蝇。在过去几年中,
首席调查员已经确定了控制哪里的因素
唾液腺会形成,而唾液腺中的细胞数量
命运。在未来数年,拟议的研究将提供以下资料:
这些因素如何联合作用来控制唾液腺的表达
基因。首席调查员也有可能是最大的一首单曲
在特定组织中表达的基因的集合。她的目标是准确定位和
描述在唾液腺发育早期起作用的基因,
尤其是协调主要形态发生运动所需的基因
使唾液腺内化,形成功能性上皮管。至
为了实现这些目标,提出了四个具体目标。在目标1中,研究是
建议测试三个早期唾液腺基因是否通过
转录因子SCR、EXD和HTH是直接的,如果是这样的话,将学习如何
TSH、ABD-B和DPP-信号传导阻断激活。在目标2中,提出了研究建议
为探讨两个早期转录因子Fkh和hkb在
唾液腺内化。FKH突变体未能完成内化
唾液腺和广泛的唾液细胞死亡。香港银行变种人启动
在错误的位置内化并形成形状异常的唾液腺。
在目标3中,建议进行研究以解决两个基因的作用,rib和
D-SemaII,在唾液腺的位置。肋骨突变体存在缺陷
唾液腺细胞的定向迁移和D-SemaII突变体的远端
唾液腺的一部分位置异常。在目标4中,研究是
提出寻找唾液腺形态发生所需的新基因。这些新的
基因将从首席调查员收集的
已知在发育中的唾液腺和EMS中表达的基因
饱和屏幕。建议的研究将提供工作蓝图,以
所有高等生物体中器官形成的早期事件。
项目成果
期刊论文数量(0)
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Deborah J Andrew其他文献
Deborah J Andrew的其他文献
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{{ truncateString('Deborah J Andrew', 18)}}的其他基金
GPCR signaling during embryonic organ formation
胚胎器官形成过程中的 GPCR 信号传导
- 批准号:
10584164 - 财政年份:2023
- 资助金额:
$ 26.85万 - 项目类别:
Coordination of Growth and Form in the Embryonic Salivary Gland and Trachea
胚胎唾液腺和气管生长和形态的协调
- 批准号:
10453482 - 财政年份:2021
- 资助金额:
$ 26.85万 - 项目类别:
Generation of transmission-compromised mosquitoes
传播受限的蚊子的产生
- 批准号:
10039237 - 财政年份:2020
- 资助金额:
$ 26.85万 - 项目类别:
2015 Salivary Glands and Exocrine Biology Gordon Research Conference
2015年唾液腺与外分泌生物学戈登研究会议
- 批准号:
8830753 - 财政年份:2015
- 资助金额:
$ 26.85万 - 项目类别: