COPAS SELECT Embryo Sorter

COPAS SELECT 胚胎分选机

• 批准号: 7586478
• 负责人: Deborah J Andrew
• 金额: $ 19.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7586478
• 关键词: Animal Model; Area; Biochemistry; Caenorhabditis elegans; Cellular biology; Charge; Computational Molecular Biology; Development; Disease; Drosophila genus; Drosophila melanogaster; Education; Embryo; Event; Experimental Models; Fertilization; Funding; Genetic Screening; Genotype; Germ Cells; Grant; Housing; Human Biology; Individual; Injury; Kidney; Laboratories; Learning; Maintenance; Manuals; Mediating; Minor; Molecular; Morphogenesis; Myoblasts; Nematoda; Neurons; Organ; Organism; Physiology; Procedures; Replacement Therapy; Research; Research Training; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Sensory; Signal Pathway; Signal Transduction; Site; Sorting - Cell Movement; Specialist; Specific qualifier value; Techniques; Tissues; Training; Training and Education; Translational Repression; United States National Institutes of Health; Universities; Work; cell motility; direct application; experience; instrument; instrumentation; neuronal cell body; programs; public health relevance; research study; sexual dimorphism; therapeutic target

DESCRIPTION (provided by applicant): Developmental biologists from several departments throughout Johns Hopkins University are submitting this application to acquire a COPAS Select Embryo Sorter for use in research and education in studies of Drosophila melanogaster and Caenorhabditis elegans. Historically, these organisms have had a significant impact on our understanding of human biology; a major experimental limitation, however, has been large-scale sorting of organisms of distinct genotypes. Although manual sorting techniques are successfully employed to obtain small samples, this procedure is too inefficient to isolate enough tissue for many applications, including microarray analyses, quantitative RT-PCR and biochemistry, or to use for large-scale genetic screens. Thus, we are applying for funds through the NIH Shared Instrumentation Grant Program (S10) to purchase, maintain and train individuals to use the COPAS Select Embryo Sorter from Union Biometrica. This is the only commercially available instrument of this kind. We have assembled a group of five major users, five minor users and a collaborator from across Johns Hopkins University who work in areas of developmental, molecular and computational biology, and whose research would be substantially enriched by the acquisition of this instrument. The users will focus on questions related to (1) deciphering the transcriptional networks controlling organ morphogenesis and physiology, (2) discovering how sexual dimorphism is controlled in both the germline and soma, (3) learning how germ cells are specified, maintained and, ultimately, differentiate (4) elucidating the signaling pathways controlling axonal targeting and sensory signaling, (5) understanding the cellular and molecular components mediating directed cell migration, (6) revealing the molecular constituents and events controlling myoblast fusion, and (7) deciphering the mechanisms of microRNA-mediated translational repression. This instrument will allow each user to carry out experiments that would otherwise be impossible and will also provide a mechanism for the cross-fertilization of ideas and the augmentation of research training and education. The embryo sorter will be housed in common space in the Cell Biology Department, adjacent to the laboratory of the PI on this grant. This site can be easily accessed by all of the users and is near several individuals who have experience using an earlier version of the instrument at other universities. Moreover, a research specialist in the PI's laboratory will be in charge of routine maintenance and training all new users. Public Health Relevance: Model organisms have become increasingly important in revealing the molecular and cellular mechanisms underlying normal development and disease. The studies included in this application are directed toward understanding key developmental events in organ and germ cell specification and differentiation in two experimental model organisms: the fruit fly Drosophila melanogaster and the nematode worm, Caenorhabditis elegans. Molecules and mechanisms discovered through these studies will provide therapeutic targets for treatment of diseases of the vasculature and kidneys, for reestablishing functional neuronal connections after traumatic injury, and for organ replacement therapy.

描述(由申请人提供)：来自约翰霍普金斯大学多个系的发育生物学家正在提交这份申请，以获得一台Copas精选胚胎分选器，用于研究和教育果蝇和秀丽线虫。从历史上看，这些生物对我们对人类生物学的理解产生了重大影响；然而，一个主要的实验限制是对不同基因类型的生物进行大规模分类。虽然人工分选技术被成功地用来获得小样本，但这种方法效率太低，无法分离出足够的组织来用于许多应用，包括微阵列分析、定量RT-PCR和生物化学，或者用于大规模的基因筛查。因此，我们正在通过NIH共享仪器赠款计划(S10)申请资金，以购买、维护和培训个人使用联合生物识别公司的Copas精选胚胎分选器。这是唯一可以在商业上买到的这类仪器。我们已经组建了一个由来自约翰·霍普金斯大学的五名主要用户、五名次要用户和一名合作者组成的小组，他们在发育、分子和计算生物学领域工作，他们的研究将因获得这台仪器而大大丰富。用户将专注于与以下问题有关的问题：(1)破译控制器官形态发生和生理的转录网络，(2)发现生殖细胞和胞体中如何控制性二型性，(3)了解生殖细胞是如何被指定、维持并最终分化的，(4)阐明控制轴突靶向和感觉信号的信号通路，(5)了解介导定向细胞迁移的细胞和分子成分，(6)揭示控制成肌细胞融合的分子成分和事件，以及(7)破译microRNA介导的翻译抑制的机制。这一工具将使每个用户能够进行原本不可能进行的实验，还将提供一种交流思想和加强研究、培训和教育的机制。胚胎分选机将安置在细胞生物系的公共空间内，毗邻这笔赠款的PI实验室。所有用户都可以很容易地访问该网站，该网站靠近几个有在其他大学使用该仪器早期版本的经验的人。此外，PI实验室的一名研究专家将负责日常维护和培训所有新用户。公共卫生相关性：模式生物在揭示正常发育和疾病背后的分子和细胞机制方面变得越来越重要。本申请中包含的研究旨在了解两种实验模式生物：果蝇黑腹果蝇和线虫秀丽线虫在器官和生殖细胞指定和分化方面的关键发育事件。通过这些研究发现的分子和机制将为血管和肾脏疾病的治疗、创伤后重建功能性神经元连接和器官替代治疗提供治疗靶点。