COPAS SELECT Embryo Sorter

COPAS SELECT 胚胎分选机

• 批准号: 7586478
• 负责人: Deborah J Andrew
• 金额: $ 19.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7586478
• 关键词: Animal Model; Area; Biochemistry; Caenorhabditis elegans; Cellular biology; Charge; Computational Molecular Biology; Development; Disease; Drosophila genus; Drosophila melanogaster; Education; Embryo; Event; Experimental Models; Fertilization; Funding; Genetic Screening; Genotype; Germ Cells; Grant; Housing; Human Biology; Individual; Injury; Kidney; Laboratories; Learning; Maintenance; Manuals; Mediating; Minor; Molecular; Morphogenesis; Myoblasts; Nematoda; Neurons; Organ; Organism; Physiology; Procedures; Replacement Therapy; Research; Research Training; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Sensory; Signal Pathway; Signal Transduction; Site; Sorting - Cell Movement; Specialist; Specific qualifier value; Techniques; Tissues; Training; Training and Education; Translational Repression; United States National Institutes of Health; Universities; Work; cell motility; direct application; experience; instrument; instrumentation; neuronal cell body; programs; public health relevance; research study; sexual dimorphism; therapeutic target

DESCRIPTION (provided by applicant): Developmental biologists from several departments throughout Johns Hopkins University are submitting this application to acquire a COPAS Select Embryo Sorter for use in research and education in studies of Drosophila melanogaster and Caenorhabditis elegans. Historically, these organisms have had a significant impact on our understanding of human biology; a major experimental limitation, however, has been large-scale sorting of organisms of distinct genotypes. Although manual sorting techniques are successfully employed to obtain small samples, this procedure is too inefficient to isolate enough tissue for many applications, including microarray analyses, quantitative RT-PCR and biochemistry, or to use for large-scale genetic screens. Thus, we are applying for funds through the NIH Shared Instrumentation Grant Program (S10) to purchase, maintain and train individuals to use the COPAS Select Embryo Sorter from Union Biometrica. This is the only commercially available instrument of this kind. We have assembled a group of five major users, five minor users and a collaborator from across Johns Hopkins University who work in areas of developmental, molecular and computational biology, and whose research would be substantially enriched by the acquisition of this instrument. The users will focus on questions related to (1) deciphering the transcriptional networks controlling organ morphogenesis and physiology, (2) discovering how sexual dimorphism is controlled in both the germline and soma, (3) learning how germ cells are specified, maintained and, ultimately, differentiate (4) elucidating the signaling pathways controlling axonal targeting and sensory signaling, (5) understanding the cellular and molecular components mediating directed cell migration, (6) revealing the molecular constituents and events controlling myoblast fusion, and (7) deciphering the mechanisms of microRNA-mediated translational repression. This instrument will allow each user to carry out experiments that would otherwise be impossible and will also provide a mechanism for the cross-fertilization of ideas and the augmentation of research training and education. The embryo sorter will be housed in common space in the Cell Biology Department, adjacent to the laboratory of the PI on this grant. This site can be easily accessed by all of the users and is near several individuals who have experience using an earlier version of the instrument at other universities. Moreover, a research specialist in the PI's laboratory will be in charge of routine maintenance and training all new users. Public Health Relevance: Model organisms have become increasingly important in revealing the molecular and cellular mechanisms underlying normal development and disease. The studies included in this application are directed toward understanding key developmental events in organ and germ cell specification and differentiation in two experimental model organisms: the fruit fly Drosophila melanogaster and the nematode worm, Caenorhabditis elegans. Molecules and mechanisms discovered through these studies will provide therapeutic targets for treatment of diseases of the vasculature and kidneys, for reestablishing functional neuronal connections after traumatic injury, and for organ replacement therapy.

描述（由申请人提供）：来自约翰·霍普金斯大学多个系的发育生物学家正在提交此申请，以获取 COPAS Select 胚胎分选机，用于果蝇和秀丽隐杆线虫的研究和教育。从历史上看，这些生物体对我们对人类生物学的理解产生了重大影响。然而，一个主要的实验限制是对不同基因型的生物体进行大规模分类。尽管手动分选技术已成功用于获取小样本，但该程序效率太低，无法分离出足够的组织用于许多应用，包括微阵列分析、定量 RT-PCR 和生物化学，或用于大规模遗传筛选。因此，我们正在通过 NIH 共享仪器拨款计划 (S10) 申请资金，以购买、维护和培训个人使用 Union Biometrica 的 COPAS Select 胚胎分选仪。这是同类中唯一的市售仪器。我们聚集了一个由五位主要用户、五位次要用户和一位来自约翰·霍普金斯大学的合作者组成的小组，他们在发育、分子和计算生物学领域工作，购买该仪器将大大丰富他们的研究。用户将重点关注与以下相关的问题：(1) 破译控制器官形态发生和生理学的转录网络，(2) 发现生殖系和体细胞中性二态性是如何控制的，(3) 了解生殖细胞如何被指定、维持并最终分化 (4) 阐明控制轴突靶向和感觉信号传导的信号传导途径，(5) 了解介导的细胞和分子成分 定向细胞迁移，(6) 揭示控制成肌细胞融合的分子成分和事件，(7) 破译 microRNA 介导的翻译抑制机制。该仪器将允许每个用户进行原本不可能进行的实验，并且还将提供一种思想交叉传播和加强研究培训和教育的机制。胚胎分选机将安置在细胞生物学系的公共空间内，毗邻本次资助的 PI 实验室。所有用户都可以轻松访问该网站，并且该网站附近有几个在其他大学有使用该仪器早期版本经验的人。此外，PI实验室的研究专家将负责日常维护和培训所有新用户。公共卫生相关性：模型生物在揭示正常发育和疾病的分子和细胞机制方面变得越来越重要。本申请中包含的研究旨在了解两种实验模型生物（果蝇果蝇和线虫秀丽隐杆线虫）的器官和生殖细胞规范和分化的关键发育事件。通过这些研究发现的分子和机制将为治疗脉管系统和肾脏疾病、重建创伤后功能性神经元连接以及器官替代疗法提供治疗靶点。