PURCHASE OF 600MHZ NMR SPECTROMETER

要求提供600 MHz NMR光谱仪的资金，以支持UCLA的NIH和NSF资助的研究人员的研究。光谱仪将位于UCLA-DOE大分子NMR设施内。这款高场NMR光谱仪将用于帮助解决主要用户提出的蛋白质、核酸、DNA-蛋白质、RNA-蛋白质和蛋白质-蛋白质复合物的结构。五个主要用户的研究项目用以下标题加以说明：（1）核酸和复合物的结构研究（Feigon），（2）蛋白质及其DNA复合物的结构研究（Clubb），（3）Vpr相关蛋白质的结构/功能（Chen），（4）DNA结构蛋白质的结构研究（约翰逊），（5）嗜气火杆菌和结核分枝杆菌蛋白质溶液结构的研究。该谱仪是迫切需要提供足够的高场核磁共振时间的主要用户提出的项目，以及允许额外的小用户更多地访问高场核磁共振谱仪的大分子研究。

Funding is requested for a 600 MHz NMR spectrometer to support the research of a group of NIH and NSF funded researchers at UCLA. The spectrometer will be located in the UCLA-DOE macromolecular NMR facility. This high field NMR spectrometer will be used to help solve the structures of proteins, nucleic acids, DNA-protein, RNA-protein, and protein-protein complexes proposed for investigation by the major users. The research projects of the five major users are described with the following titles: (1) Structural studies of nucleic acids and complexes (Feigon), (2) Structural studies of proteins and their DNA complexes (Clubb), (3) Structure/Function of Vpr Associated Proteins (Chen), (4) Structural studies of DNA architectural proteins (Johnson), and (5) Structural studies in solution of proteins from pyrobaculum aerophilum and mycobacterium tuberculosis (Eisenberg). This spectrometer is urgently needed to provide adequate high field NMR time for the projects proposed by the major users, as well as to allow additional minor users more access to high field NMR spectrometers for macromolecular studies.