TRAINING IN USE OF DMX ELECTRONICS

将继续研究这一机制的结构和机制， 四氢蝶呤依赖性羟化酶。 调节和催化 酪氨酸、苯丙氨酸和色氨酸羟化酶的结构域具有 在E.杆菌 结构测定 用X射线衍射法测定酪氨酸的催化结构域 羟化酶在不存在和存在底物或底物 将完成模拟。 磷酸化对细胞凋亡的影响 酪氨酸羟化酶的调节结构域的结构将是 通过使用高场NMR方法测定。 嵌合蛋白 含有调节和催化突变体的基因组将基于 这些结构。 的调节和催化特性， 将确定突变蛋白。

Studies will continue on the structures and mechanisms of the tetrahydropterin dependent hydroxylases. The regulatory and catalytic domains of tyrosine, phenylalanine, and tryptophan hydroxylase have all been expressed separately in E. coli. The structure determination by X-ray diffraction methods of the catalytic domain of tyrosine hydroxylase in the absence and presence of substrates or substrate analogs will be completed. The effects of phosphorylation on the structure of the regulatory domain of tyrosine hydroxylase will be determined by using high field NMR methods. Chimeric proteins containing regulatory and catalytic mutants will be constructed based on these structures. The regulatory and catalytic properties of the mutant proteins will be determined.