HIV: NMR STUDIES OF 15N, 13C LABELED RIBONUCLEOTIDES

HIV：15N、13C 标记核糖核苷酸的 NMR 研究

• 批准号: 6120831
• 负责人: JULI FEIGON
• 金额: $ 9.71万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-15 至 2000-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120831
• 关键词: biomedical resource; enzymes; virus

The SIR provided D,L-[15 N2-imidazoyl]Histidine; 1.48g Cleland and Kreevoy recently advanced the idea that a special type of hydrogen bond (H-bond), termed a low-barrier hydrogen bond (LBHB), may account for the "missing" transition state stabilization underlying the catalytic power of many enzymes, and Frey et al. have proposed that the H-bond between aspartic acid 102 and histidine 57 in the catalytic triad of serine proteases is an example of a catalytically important LBHB. Using "N labeled histidine provided by the SIR and NMR spectroscopy, we have shown that the aspartic acid-histidine and cis urocanic H-bonds that are inconsistent with fundamental tenets of the LBHB hypothesis. The inconsistencies between theory and experiment in these paradigm systems cast doubt on the existence of , as currently defined, within enzyme active sites.

SIR提供D，L-[15 n2 -咪唑基]组氨酸；1.48克克莱兰和