ULTRAFAST MULTIPHOTON LASER ACTIVATION SYSTEM

超快多光子激光激活系统

基本信息

  • 批准号:
    6291410
  • 负责人:
  • 金额:
    $ 37.46万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2001
  • 资助国家:
    美国
  • 起止时间:
    2001-05-01 至 2002-04-30
  • 项目状态:
    已结题

项目摘要

The objective of this proposal is to create an Ultrafast Multiphoton Laser Activation System. This integrated laser instrument system is to be assembled to support a select set of innovative biomedical and biotechnology research programs that are implemented by multiphoton photochemistry and photophysics. Ultrafast (approximately 100 femtosecond) pulses generated by mode-locked lasers allow simultaneous absorption of two or more photons from bright pulsed illumination for molecular excitation as in the multiphoton laser scanning fluorescence microscopy used in biomedical research in living tissue. However, research on multiphoton excitation is evolving powerful photochemical tools for molecular biology and biomedical research and diagnosis. In particular, multiphoton photochemical crosslinking of the DNA of specific genes to the corresponding regulatory proteins allows assay of the molecular biology of these essential regulators of gene expression and identification of each particular regulatory enzyme-DNA pairing. The proposed laser system provides the appropriate, bright, ultrafast pulses and pulse pairs at ultraviolet (approximately 250) wavelengths to implement efficiently this crosslinking process in vitro and, it is expected, in vivo. The high-energy femtosecond pulses of the Ultrafast Laser System are provided throughout the wide range from 210 nm to 3,000 nm needed to enable or enhance the entire group of projects. Other user's applications of the Ultrafast Laser System include (1) development of caged reagents and caged neurotransmitters for localized micro-pharmacology by multiphoton photoactivation, (2) research on oxidative stress effects in disease by multiphoton oxidant activation of molecules in tissue, (3) investigations of biological photodamage mechanisms, (4) developments of several biophysical technologies, and (5) research on the photophysical mechanisms of all of the relevant multiphoton photochemical processes involved. Several biophysical technology developments are facilitated by the proposed instrument system, in particular, development of a multiphoton photochemical method to fabricate 3-dimensionally complex microfluidic systems for biotechnology devices, and photophysics for enhanced implementation of multiphoton microscopy and for biomedical endoscopic spectroscopy of intrinsic tissue fluorescence to recognize disease states. The instrument system consists of a tunable titanium:sapphire laser oscillator, regenerative amplifier, two independently tunable optical parametric oscillators, harmonic generators for frequency multiplication, delay lines and associated instrumentation in order to encompass the high pulse energy and unusually broad tuning range requirements of the select biomedical user community and to safely provide user friendly instrumentation for the biological user community.
本提案的目的是建立一个超快多光子激光激活系统。这种集成的激光仪器系统将被组装,以支持一套创新的生物医学和生物技术研究计划,这些计划是由多光子光化学和光物理学实施的。锁模激光器产生的超快(约100飞秒)脉冲允许同时吸收两个或多个来自明亮脉冲照明的光子,用于分子激发,如在活组织生物医学研究中使用的多光子激光扫描荧光显微镜。然而,对多光子激发的研究正在为分子生物学和生物医学研究和诊断发展强大的光化学工具。特别是,特定基因的DNA与相应的调节蛋白的多光子光化学交联允许测定这些基因表达的基本调节因子的分子生物学和鉴定每个特定的调节酶-DNA配对。所提出的激光系统提供了适当的,明亮的,超快的脉冲和脉冲对在紫外线(约250)波长,有效地实现这种交联过程在体外,它是预期的,在体内。超快激光系统的高能飞秒脉冲可在210 nm至3,000 nm的范围内提供,以支持或增强整个项目组。超快激光系统的其他用户应用包括(1)通过多光子光活化开发用于局部微药理学的笼状试剂和笼状神经递质,(2)通过组织中分子的多光子氧化剂活化研究疾病中的氧化应激效应,(3)研究生物光损伤机制,(4)开发几种生物物理技术,(5)研究所有相关的多光子光化学过程的光物理机制。几个生物物理技术的发展,促进了拟议的仪器系统,特别是多光子光化学方法的发展,以制造三维复杂的微流体系统的生物技术设备,和生物物理学增强实施的多光子显微镜和生物医学内窥镜光谱的内在组织荧光识别疾病状态。该仪器系统包括一个可调钛:蓝宝石激光振荡器,再生放大器,两个独立的可调光学参量振荡器,倍频谐波发生器,延迟线和相关的仪器,以涵盖高脉冲能量和异常宽的调谐范围的要求选择生物医学用户社区和安全地提供用户友好的仪器为生物用户社区。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(1)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

WATT W WEBB其他文献

WATT W WEBB的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('WATT W WEBB', 18)}}的其他基金

Development of Medical Multiphoton Microscopic Endoscopy
医用多光子显微内窥镜的发展
  • 批准号:
    7425824
  • 财政年份:
    2007
  • 资助金额:
    $ 37.46万
  • 项目类别:
Development of Medical Multiphoton Microscopic Endoscopy
医用多光子显微内窥镜的发展
  • 批准号:
    7596376
  • 财政年份:
    2007
  • 资助金额:
    $ 37.46万
  • 项目类别:
Development of Medical Multiphoton Microscopic Endoscopy
医用多光子显微内窥镜的发展
  • 批准号:
    7800442
  • 财政年份:
    2007
  • 资助金额:
    $ 37.46万
  • 项目类别:
Development of Medical Multiphoton Microscopic Endoscopy
医用多光子显微内窥镜的发展
  • 批准号:
    7172760
  • 财政年份:
    2007
  • 资助金额:
    $ 37.46万
  • 项目类别:
Development of Medical Multiphoton Microscopic Endoscopy
医用多光子显微内窥镜的发展
  • 批准号:
    8048113
  • 财政年份:
    2007
  • 资助金额:
    $ 37.46万
  • 项目类别:
Nascent Dynamic Intermediates in Amyloid Aggregation
淀粉样蛋白聚集中的新生动态中间体
  • 批准号:
    6963283
  • 财政年份:
    2005
  • 资助金额:
    $ 37.46万
  • 项目类别:
Nascent Dynamic Intermediates in Amyloid Aggregation
淀粉样蛋白聚集中的新生动态中间体
  • 批准号:
    7140349
  • 财政年份:
    2005
  • 资助金额:
    $ 37.46万
  • 项目类别:
CONSULTATIONS ON APPLICATIONS OF MULTI PHOTON MICROSCOPY
多光子显微镜应用咨询
  • 批准号:
    6349480
  • 财政年份:
    2000
  • 资助金额:
    $ 37.46万
  • 项目类别:
INVITED LECTURE SOMETHING NEW IN MICROSCOPY
受邀讲座显微镜新内容
  • 批准号:
    6349463
  • 财政年份:
    2000
  • 资助金额:
    $ 37.46万
  • 项目类别:
PHONE CONSULTATION: IMAGING IN RAT BRAIN (10 & 20 26 28 29 & 98, 4 & 15 & 99)
电话咨询:大鼠脑部成像 (10
  • 批准号:
    6349475
  • 财政年份:
    2000
  • 资助金额:
    $ 37.46万
  • 项目类别:

相似海外基金

Tuneable short-wavelength infrared mode-locked fibre lasers
可调谐短波长红外锁模光纤激光器
  • 批准号:
    EP/Y001915/1
  • 财政年份:
    2024
  • 资助金额:
    $ 37.46万
  • 项目类别:
    Research Grant
CAREER: Integrated Lithium Niobate Femtosecond Mode-Locked Lasers and Ultrafast Photonic Systems
职业:集成铌酸锂飞秒锁模激光器和超快光子系统
  • 批准号:
    2338798
  • 财政年份:
    2024
  • 资助金额:
    $ 37.46万
  • 项目类别:
    Continuing Grant
MEMS-metasurface Based Tunable Optical Vortex Lasers for smart free-space communication
用于智能自由空间通信的基于 MEMS 超表面的可调谐光学涡旋激光器
  • 批准号:
    EP/X034542/2
  • 财政年份:
    2024
  • 资助金额:
    $ 37.46万
  • 项目类别:
    Research Grant
Investigating spatio-temporal instabilities in next-generation lasers
研究下一代激光器的时空不稳定性
  • 批准号:
    FT230100388
  • 财政年份:
    2024
  • 资助金额:
    $ 37.46万
  • 项目类别:
    ARC Future Fellowships
RII Track-4: NSF: Development of Semiconductor Lasers and Passive Devices on a Single Sapphire Platform for Integrated Microwave Photonics
RII Track-4:NSF:在单个蓝宝石平台上开发用于集成微波光子学的半导体激光器和无源器件
  • 批准号:
    2327229
  • 财政年份:
    2024
  • 资助金额:
    $ 37.46万
  • 项目类别:
    Standard Grant
Lasers for quantum-enabled position, navigation, and timing technologies
用于量子定位、导航和授时技术的激光器
  • 批准号:
    2902868
  • 财政年份:
    2024
  • 资助金额:
    $ 37.46万
  • 项目类别:
    Studentship
Infra-Plas: Colloidal Quantum Dots for Short-Wave Infrared Plasmonic Lasers
Infra-Plas:用于短波红外等离子激光器的胶体量子点
  • 批准号:
    EP/Z000912/1
  • 财政年份:
    2024
  • 资助金额:
    $ 37.46万
  • 项目类别:
    Fellowship
Integrated Solid-State Steerable Lasers (I-STEER)
集成固态可控激光器 (I-STEER)
  • 批准号:
    EP/X032868/1
  • 财政年份:
    2024
  • 资助金额:
    $ 37.46万
  • 项目类别:
    Research Grant
Integrated Solid-State Steerable Lasers (I-STEER)
集成固态可控激光器 (I-STEER)
  • 批准号:
    EP/X03299X/1
  • 财政年份:
    2024
  • 资助金额:
    $ 37.46万
  • 项目类别:
    Research Grant
Lasers for Accelerated Net-Zero Transition-Lasers4NetZero
用于加速净零过渡的激光器-Lasers4NetZero
  • 批准号:
    EP/Y030591/1
  • 财政年份:
    2024
  • 资助金额:
    $ 37.46万
  • 项目类别:
    Research Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了