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Development of Medical Multiphoton Microscopic Endoscopy

医用多光子显微内窥镜的发展

基本信息

批准号：
8048113
负责人：
WATT W WEBB
金额：
$ 102.65万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-06-01 至 2013-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8048113
关键词：
3-Dimensional Address Anatomy Animals Area Atlases Australia Back Biological Biomedical Engineering Biophysics Biopsy Biopsy Specimen Biotechnology Caliber Cancer Diagnostics Cells Cellular biology Characteristics Clinical Clinical Medicine Collaborations Collagen Colonoscopy Color Complex Conceptions Confocal Microscopy Coupling Custom Data Databases Detection Development Devices Diagnosis Diagnostic Diagnostic Imaging Disadvantaged Disease Distal Documentation Effectiveness Endoscopes Endoscopy Engineering Ensure Evaluation Feedback Fiber Fiber Optics Fluorescence Foundations Funding Gastroenterology Generations Grant Health Healthcare Hearing Histology Human Human Resources Human body Image Image Analysis Imaging technology In Situ Industry Intellectual Property Japan Label Laboratories Laboratory Research Laser Scanning Microscopy Lasers Lead Legal patent Length Libraries Life Light Lighting Literature Location Lung nodule Malignant Neoplasms Malignant neoplasm of ovary Manufacturer Name Mechanics Medical Medical Imaging Medical Technology Medicine Metabolism Methodology Methods Microscope Microscopic Microscopy Microtubule Bundle Miniaturization Modern Medicine Motivation Mus Nanostructures National Institute of Biomedical Imaging and Bioengineering Nature Needle biopsy procedure Neurodegenerative Disorders Neurosciences New York City Occupations Operative Surgical Procedures Optics Organ Participant Pathologist Pathology Pathway interactions Patients Physicians Physics Physiologic pulse Physiology Postdoctoral Fellow Preparation Procedures Production Property Public Health Publications Publishing Qualifying Quality Control Reporting Research Research Support Reservations Resolution Resources Robotics Sampling Sapphire Scanning Scientist Services Signal Transduction Silicon Dioxide Solid Source Spectrum Analysis Staging Staining method Stains Structure Supervision Surface Surgeon Surveys System Systems Development Techniques Technology Technology Transfer Testing Time Tissues Tokyo Training Transgenic Mice Transgenic Model Uncertainty United States National Institutes of Health Universities Validation Work absorption base cancer imaging clinical Diagnosis design digital direct application evidence base experience flexibility follow-up human tissue in vivo innovation instrument instrumentation interest light transmission medical schools medical specialist medical specialties miniaturize optical communication optical fiber optical imaging professor progenitor programs quantum research and development response robotic device second harmonic success technology development tissue fixing tool tool development urologic user-friendly

项目摘要

DESCRIPTION (provided by applicant): The ultimate objective of this Bioengineering Research Partnership is creation of significant advances in the functional effectiveness of medical endoscopy for public health care by enabling the real-time, in situ incorporation of the biophysical micro-imaging of the intrinsic diagnostic properties of tissue in vivo. The fluorescence and second harmonic generation in tissue images by multiphoton microscopy (MPM) without the need for extrinsic labeling are expected to enable effective medical utilization of the capabilities of MPM for in vivo detection and diagnostic recognition of maladies such as cancers, collagen structure damage, and fibrotic disease states, etc., by MPM Endoscopy. MPM Endoscopy will utilize the MPM imaging capabilities discovered and analyzed in biophysical laboratory research, including living preparations, and transgenic model animals displaying various disease states - with validation by histology. MPM was invented and continues to be advanced through associated research programs of the PI and key personnel at Cornell University, and now with Weill Medical College of Cornell University. The first essential aim is development of the data foundation for use in human medicine with MPM images of fresh medical biopsies compared to their appraisal and validation by collaborating pathologists' histology and clinical diagnoses, leading to production of atlases of diagnostic MPM images at Weill Medical's laboratory for eventual dissemination. To accomplish this transition of the MPM tissue imaging capabilities from the laboratory to medical application via MPM endoscopy, a representative cadre of physicians and surgeons at Cornell Weill Medical have assembled to partner in testing and evaluating this capability in their diverse specialties. Simultaneously, an essential aim is development of hyper-miniaturized, reliable MPM endoscopic apparati compatible with the diverse geometries required for various organs of human physiology, which can progress in parallel with more accessible designs for equipping of larger scale endoscopies. Despite published progenitors, instrumentation comprises the major developmental challenge of the program. To accomplish this objective, innovative research by our key personnel with established expertise in ultrafast optics and nanostructure fabrication is invoked to design, fabricate and test potential systems. Biomedical engineering of microscopy components installed in endoscopy apparati is required for testing, ultimately in human MPM Endoscopy applications initially by our collaborating MD's. Finally, technology transfer to commercial manufacturers to provide medical instrument availability is required to achieve the ultimate objective of this: new medical capability.

描述（由申请人提供）：该生物工程研究伙伴关系的最终目标是通过实时、原位结合体内组织内在诊断特性的生物物理显微成像，在医疗内窥镜用于公共卫生保健的功能有效性方面取得重大进展。通过多光子显微术（MPM）在组织图像中产生荧光和二次谐波而不需要外部标记，预期使得能够有效地医学利用MPM的能力，用于诸如癌症、胶原结构损伤和纤维化疾病状态等疾病的体内检测和诊断识别，通过MPM内窥镜检查。MPM内窥镜将利用在生物物理实验室研究中发现和分析的MPM成像能力，包括活体制剂和显示各种疾病状态的转基因模型动物-通过组织学验证。MPM是通过PI和康奈尔大学关键人员的相关研究项目发明的，并继续得到推进，现在与康奈尔大学威尔医学院合作。第一个基本目标是开发用于人类医学的数据基础，与新鲜医学活检的MPM图像进行比较，通过合作病理学家的组织学和临床诊断进行评估和验证，从而在Weill Medical实验室生产诊断MPM图像的地图集，以最终传播。为了通过MPM内窥镜完成MPM组织成像能力从实验室到医疗应用的过渡，Cornell Weill Medical的一批具有代表性的内科医生和外科医生聚集在一起，在他们的不同专业中测试和评估这种能力。同时，一个基本目标是开发超小型化的、可靠的MPM内窥镜装置，其与人体生理学的各种器官所需的不同几何形状兼容，这可以与用于装备更大规模内窥镜的更容易接近的设计并行地发展。尽管已发表的祖先，仪器包括该计划的主要发展挑战。为了实现这一目标，我们的关键人员在超快光学和纳米结构制造方面的专业知识被用来设计，制造和测试潜在的系统。安装在内窥镜设备中的显微镜组件的生物医学工程需要进行测试，最终由我们的合作MD在人类MPM内窥镜应用中进行测试。最后，需要向商业制造商转让技术，以提供医疗仪器，以实现这一最终目标：新的医疗能力。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Three-photon excited fluorescence imaging of unstained tissue using a GRIN lens endoscope.

DOI：
10.1364/boe.4.000652
发表时间：
2013-05-01
期刊：
Biomedical optics express
影响因子：
3.4
作者：
Huland DM;Charan K;Ouzounov DG;Jones JS;Nishimura N;Xu C
通讯作者：
Xu C

Multiphoton microscopy of prostate and periprostatic neural tissue: a promising imaging technique for improving nerve-sparing prostatectomy.

前列腺和前列腺周围神经组织的多光子显微镜：一种有前途的成像技术，用于改善保留神经的前列腺切除术。

DOI：
10.1089/end.2009.0221
发表时间：
2009
期刊：
Journal of endourology
影响因子：
2.7
作者：
Yadav,Rajiv;Mukherjee,Sushmita;Hermen,Michael;Tan,Gerald;Maxfield,FrederickR;Webb,WattW;Tewari,AshutoshK
通讯作者：
Tewari,AshutoshK

Mechanisms of quenching of Alexa fluorophores by natural amino acids.

DOI：
10.1021/ja100500k
发表时间：
2010-06-02
期刊：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
影响因子：
15
作者：
Chen, Huimin;Ahsan, Syed S.;Santiago-Berrios, Mitk'El B.;Abruna, Hector D.;Webb, Watt W.
通讯作者：
Webb, Watt W.

Miniature varifocal objective lens for endomicroscopy.

用于内窥镜检查的微型变焦物镜。