Development of Medical Multiphoton Microscopic Endoscopy

医用多光子显微内窥镜的发展

• 批准号: 7425824
• 负责人: WATT W WEBB
• 金额: $ 105.49万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7425824
• 关键词: 3-Dimensional; Address; Anatomy; Animals; Area; Atlases; Australia; Back; Biological; Biomedical Engineering; Biophysics; Biopsy; Biopsy Specimen; Biotechnology; Caliber; Cancer Diagnostics; Caring; Cells; Cellular biology; Characteristics; Classification; Clinical; Clinical Medicine; Collaborations; Collagen; Colonoscopy; Color; Commercial Sources; Compatible; Complex; Computer Systems Development; Conceptions; Confocal Microscopy; Coupling; Custom; Data; Databases; Depth; Detection; Development; Devices; Diagnosis; Diagnostic; Diagnostic Imaging; Disadvantaged; Disease; Distal; Documentation; Effectiveness; Endoscopes; Endoscopy; Engineering; Ensure; Evaluation; Facility Construction Funding Category; Feedback; Fiber; Fiber Optics; Fluorescence; Foundations; Funding; Gastroenterology; Generations; Grant; Health; Hearing; Histology; Human; Human Resources; Human body; Image; Image Analysis; Imaging technology; In Situ; Industry; Intellectual Property; Japan; Label; Laboratories; Laboratory Research; Laser Scanning Microscopy; Lasers; Lead; Legal patent; Length; Libraries; Life; Light; Lighting; Literature; Location; Lung nodule; Malignant Neoplasms; Malignant neoplasm of ovary; Manufacturer Name; Mechanics; Medical; Medical Imaging; Medical Technology; Medicine; Metabolism; Methodology; Methods; Microscope; Microscopic; Microscopy; Microtubule Bundle; Miniaturization; Modern Medicine; Motivation; Mus; Nanostructures; Nature; Needle biopsy procedure; Neurodegenerative Disorders; Neurosciences; New York City; Numbers; Occupations; Operative Surgical Procedures; Optics; Organ; Participant; Pathologist; Pathology; Pathway interactions; Patients; Personal Satisfaction; Physicians; Physics; Physiologic pulse; Physiology; Postdoctoral Fellow; Preparation; Principal Investigator; Procedures; Production; Property; Public Health; Publications; Publishing; Pulse taking; Qualifying; Quality Control; Range; Reporting; Research; Research Personnel; Research Support; Reservations; Resolution; Resources; Robotics; Sampling; Sapphire; Scanning; Scientist; Services; Signal Transduction; Silicon Dioxide; Solid; Spectrum Analysis; Staging; Staining method; Stains; Standards of Weights and Measures; Structure; Supervision; Surface; Surgeon; Surveys; System; Techniques; Technology; Technology Transfer; Testing; Time; Tissues; Tokyo; Training; Transgenic Mice; Transgenic Model; Uncertainty; United States Food and Drug Administration; United States National Institutes of Health; Universities; Validation; Victoria Austrailia; Work; absorption; base; cancer imaging; clinical Diagnosis; college; design; digital; direct application; experience; follow-up; human tissue; in vivo; innovation; instrument; instrumentation; interest; light transmission; medical specialist; medical specialties; miniaturize; optical communication; optical fiber; optical imaging; professor; progenitor; programs; quantum; research and development; response; robotic device; second harmonic; success; technology development; tissue fixing; tool; tool development; urologic; user-friendly

DESCRIPTION (provided by applicant): The ultimate objective of this Bioengineering Research Partnership is creation of significant advances in the functional effectiveness of medical endoscopy for public health care by enabling the real-time, in situ incorporation of the biophysical micro-imaging of the intrinsic diagnostic properties of tissue in vivo. The fluorescence and second harmonic generation in tissue images by multiphoton microscopy (MPM) without the need for extrinsic labeling are expected to enable effective medical utilization of the capabilities of MPM for in vivo detection and diagnostic recognition of maladies such as cancers, collagen structure damage, and fibrotic disease states, etc., by MPM Endoscopy. MPM Endoscopy will utilize the MPM imaging capabilities discovered and analyzed in biophysical laboratory research, including living preparations, and transgenic model animals displaying various disease states - with validation by histology. MPM was invented and continues to be advanced through associated research programs of the PI and key personnel at Cornell University, and now with Weill Medical College of Cornell University. The first essential aim is development of the data foundation for use in human medicine with MPM images of fresh medical biopsies compared to their appraisal and validation by collaborating pathologists' histology and clinical diagnoses, leading to production of atlases of diagnostic MPM images at Weill Medical's laboratory for eventual dissemination. To accomplish this transition of the MPM tissue imaging capabilities from the laboratory to medical application via MPM endoscopy, a representative cadre of physicians and surgeons at Cornell Weill Medical have assembled to partner in testing and evaluating this capability in their diverse specialties. Simultaneously, an essential aim is development of hyper-miniaturized, reliable MPM endoscopic apparati compatible with the diverse geometries required for various organs of human physiology, which can progress in parallel with more accessible designs for equipping of larger scale endoscopies. Despite published progenitors, instrumentation comprises the major developmental challenge of the program. To accomplish this objective, innovative research by our key personnel with established expertise in ultrafast optics and nanostructure fabrication is invoked to design, fabricate and test potential systems. Biomedical engineering of microscopy components installed in endoscopy apparati is required for testing, ultimately in human MPM Endoscopy applications initially by our collaborating MD's. Finally, technology transfer to commercial manufacturers to provide medical instrument availability is required to achieve the ultimate objective of this: new medical capability.

描述(由申请人提供)：该生物工程研究伙伴关系的最终目标是通过能够实时、原位地结合体内组织的内在诊断特性的生物物理显微成像，在公共卫生保健的医疗内窥镜的功能有效性方面取得重大进展。多光子显微镜(MPM)在组织图像中产生的荧光和二次谐波不需要外在标记，有望使MPM的能力在医学上得到有效的利用，通过MPM内窥镜对疾病进行体内检测和诊断识别，如癌症、胶原结构损伤和纤维化疾病状态等。MPM内窥镜将利用在生物物理实验室研究中发现和分析的MPM成像能力，包括活的制剂和显示各种疾病状态的转基因模型动物--并经组织学验证。MPM是通过康奈尔大学的PI和关键人员的相关研究计划发明的，现在与康奈尔大学的威尔医学院合作。第一个基本目标是开发用于人类医学的数据基础，与新鲜医疗活检的MPM图像进行比较，通过与病理学家的组织学和临床诊断合作对其进行评估和验证，导致在Weill Medical的实验室制作诊断MPM图像的图集，以供最终传播。为了通过MPM内窥镜完成MPM组织成像能力从实验室到医疗应用的转变，康奈尔·威尔医疗公司的一支具有代表性的内科和外科医生团队聚集在一起，在他们的不同专业中测试和评估这一能力。同时，一个重要的目标是开发超小型、可靠的MPM内窥镜设备，与人体生理器官所需的各种几何形状兼容，可以与更容易获得的设计并行发展，以装备更大规模的内窥镜。尽管发表了前身，但仪器构成了该计划的主要发展挑战。为了实现这一目标，我们调用在超快光学和纳米结构制造方面拥有成熟专业知识的关键人员进行创新研究，以设计、制造和测试潜在的系统。安装在内窥镜设备中的显微镜组件的生物医学工程需要进行测试，最终由我们合作的MD最初在人类MPM内窥镜应用程序中进行测试。最后，需要向商业制造商转让技术以提供医疗器械可用性，以实现这一最终目标：新的医疗能力。