Development of Medical Multiphoton Microscopic Endoscopy

医用多光子显微内窥镜的发展

• 批准号: 7425824
• 负责人: WATT W WEBB
• 金额: $ 105.49万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7425824
• 关键词: 3-Dimensional; Address; Anatomy; Animals; Area; Atlases; Australia; Back; Biological; Biomedical Engineering; Biophysics; Biopsy; Biopsy Specimen; Biotechnology; Caliber; Cancer Diagnostics; Caring; Cells; Cellular biology; Characteristics; Classification; Clinical; Clinical Medicine; Collaborations; Collagen; Colonoscopy; Color; Commercial Sources; Compatible; Complex; Computer Systems Development; Conceptions; Confocal Microscopy; Coupling; Custom; Data; Databases; Depth; Detection; Development; Devices; Diagnosis; Diagnostic; Diagnostic Imaging; Disadvantaged; Disease; Distal; Documentation; Effectiveness; Endoscopes; Endoscopy; Engineering; Ensure; Evaluation; Facility Construction Funding Category; Feedback; Fiber; Fiber Optics; Fluorescence; Foundations; Funding; Gastroenterology; Generations; Grant; Health; Hearing; Histology; Human; Human Resources; Human body; Image; Image Analysis; Imaging technology; In Situ; Industry; Intellectual Property; Japan; Label; Laboratories; Laboratory Research; Laser Scanning Microscopy; Lasers; Lead; Legal patent; Length; Libraries; Life; Light; Lighting; Literature; Location; Lung nodule; Malignant Neoplasms; Malignant neoplasm of ovary; Manufacturer Name; Mechanics; Medical; Medical Imaging; Medical Technology; Medicine; Metabolism; Methodology; Methods; Microscope; Microscopic; Microscopy; Microtubule Bundle; Miniaturization; Modern Medicine; Motivation; Mus; Nanostructures; Nature; Needle biopsy procedure; Neurodegenerative Disorders; Neurosciences; New York City; Numbers; Occupations; Operative Surgical Procedures; Optics; Organ; Participant; Pathologist; Pathology; Pathway interactions; Patients; Personal Satisfaction; Physicians; Physics; Physiologic pulse; Physiology; Postdoctoral Fellow; Preparation; Principal Investigator; Procedures; Production; Property; Public Health; Publications; Publishing; Pulse taking; Qualifying; Quality Control; Range; Reporting; Research; Research Personnel; Research Support; Reservations; Resolution; Resources; Robotics; Sampling; Sapphire; Scanning; Scientist; Services; Signal Transduction; Silicon Dioxide; Solid; Spectrum Analysis; Staging; Staining method; Stains; Standards of Weights and Measures; Structure; Supervision; Surface; Surgeon; Surveys; System; Techniques; Technology; Technology Transfer; Testing; Time; Tissues; Tokyo; Training; Transgenic Mice; Transgenic Model; Uncertainty; United States Food and Drug Administration; United States National Institutes of Health; Universities; Validation; Victoria Austrailia; Work; absorption; base; cancer imaging; clinical Diagnosis; college; design; digital; direct application; experience; follow-up; human tissue; in vivo; innovation; instrument; instrumentation; interest; light transmission; medical specialist; medical specialties; miniaturize; optical communication; optical fiber; optical imaging; professor; progenitor; programs; quantum; research and development; response; robotic device; second harmonic; success; technology development; tissue fixing; tool; tool development; urologic; user-friendly

DESCRIPTION (provided by applicant): The ultimate objective of this Bioengineering Research Partnership is creation of significant advances in the functional effectiveness of medical endoscopy for public health care by enabling the real-time, in situ incorporation of the biophysical micro-imaging of the intrinsic diagnostic properties of tissue in vivo. The fluorescence and second harmonic generation in tissue images by multiphoton microscopy (MPM) without the need for extrinsic labeling are expected to enable effective medical utilization of the capabilities of MPM for in vivo detection and diagnostic recognition of maladies such as cancers, collagen structure damage, and fibrotic disease states, etc., by MPM Endoscopy. MPM Endoscopy will utilize the MPM imaging capabilities discovered and analyzed in biophysical laboratory research, including living preparations, and transgenic model animals displaying various disease states - with validation by histology. MPM was invented and continues to be advanced through associated research programs of the PI and key personnel at Cornell University, and now with Weill Medical College of Cornell University. The first essential aim is development of the data foundation for use in human medicine with MPM images of fresh medical biopsies compared to their appraisal and validation by collaborating pathologists' histology and clinical diagnoses, leading to production of atlases of diagnostic MPM images at Weill Medical's laboratory for eventual dissemination. To accomplish this transition of the MPM tissue imaging capabilities from the laboratory to medical application via MPM endoscopy, a representative cadre of physicians and surgeons at Cornell Weill Medical have assembled to partner in testing and evaluating this capability in their diverse specialties. Simultaneously, an essential aim is development of hyper-miniaturized, reliable MPM endoscopic apparati compatible with the diverse geometries required for various organs of human physiology, which can progress in parallel with more accessible designs for equipping of larger scale endoscopies. Despite published progenitors, instrumentation comprises the major developmental challenge of the program. To accomplish this objective, innovative research by our key personnel with established expertise in ultrafast optics and nanostructure fabrication is invoked to design, fabricate and test potential systems. Biomedical engineering of microscopy components installed in endoscopy apparati is required for testing, ultimately in human MPM Endoscopy applications initially by our collaborating MD's. Finally, technology transfer to commercial manufacturers to provide medical instrument availability is required to achieve the ultimate objective of this: new medical capability.

描述（由申请人提供）：该生物工程研究合作伙伴关系的最终目标是通过实时、原位整合体内组织固有诊断特性的生物物理显微成像，在公共医疗保健的医学内窥镜功能有效性方面取得重大进展。通过多光子显微镜（MPM）在组织图像中产生荧光和二次谐波，无需外在标记，预计将能够有效地利用 MPM 的功能，通过 MPM 内窥镜对癌症、胶原蛋白结构损伤和纤维化疾病状态等疾病进行体内检测和诊断识别。 MPM 内窥镜检查将利用在生物物理实验室研究中发现和分析的 MPM 成像功能，包括活体制剂和显示各种疾病状态的转基因模型动物 - 并通过组织学验证。 MPM 是通过康奈尔大学 PI 和关键人员以及现在康奈尔大学威尔医学院的相关研究项目发明并继续推进的。第一个基本目标是开发用于人类医学的数据基础，将新鲜医学活检的 MPM 图像与病理学家合作的组织学和临床诊断进行评估和验证进行比较，从而在 Weill Medical 实验室生成诊断 MPM 图像图集以供最终传播。为了通过 MPM 内窥镜实现 MPM 组织成像功能从实验室到医疗应用的转变，康奈尔威尔医疗中心的内科医生和外科医生代表骨干人员聚集在一起，在他们的不同专业中测试和评估这种能力。同时，一个基本目标是开发超小型化、可靠的 MPM 内窥镜设备，与人体生理学各个器官所需的不同几何形状兼容，这可以与用于装备更大规模内窥镜的更容易设计并行发展。尽管已发表了前身，但仪器仪表构成了该计划的主要发展挑战。为了实现这一目标，我们的关键人员在超快光学和纳米结构制造方面拥有丰富的专业知识，他们的创新研究被用来设计、制造和测试潜在的系统。内窥镜设备中安装的显微镜组件的生物医学工程需要进行测试，最终由我们的合作医学博士在人类 MPM 内窥镜应用中进行测试。最后，需要向商业制造商转让技术以提供医疗器械可用性，以实现最终目标：新的医疗能力。