PE BIOSYSTEMS VOYAGER DE MALDI-TOF

这项申请是为了获得购买Voyager-DE生物光谱测量工作站的资金。该系统使用蛋白质、多肽和寡核苷酸的基质辅助。该仪器的强大之处在于延迟提取(DE)技术。通过降低背景和提高信噪比，可以获得显著的质量分辨率。因此，对来自各种来源和脆弱分子(如DNA)的复杂蛋白质的分析可以非常准确地完成。由于减少了背景，该软件能够缩小参数范围，并显著减少进行有效分配所需的脉泽数量。该软件还可以通过搜索虚拟消化数据库来识别蛋白质。该系统是多个运营商和项目的理想之选，因为它具有通用性、广泛性和易操作性。我们的具体应用包括：C端和N端蛋白质和寡核苷酸测序，识别未知蛋白质和确定跨国后修饰。样品可以从凝胶、PVDF膜、TLC板和/或Biacore芯片等来源提取。对旅行者-DE MALDI-TOF的上瘾将有助于实现12项研究建议中详细描述的具体目标

This request is for funds to purchase a Voyager-DE Biospectrometry Workstation. This system uses matrix-assisted of proteins, peptide and oligonucleotide. The power of this instrument lies in the delayed extraction (DE) technology. Significant mass resolution is obtained by reducing the background and increasing the signal to noise ratio. Therefore analyses of complex protein matures from various sources and fragile molecules, such as DNA, can be accomplished with great accuracy. Because of the decreased background the software is able to narrow the parameters and significantly reduce the number of masers necessary to make valid assignments. The software also allows identification of proteins by searching a virtual digestion database. This system is ideal for multiple operators and projects beaus of its versatility, spread and ease of operation. Our specific applications include: C-terminal and N-terminal protein and oligonucleotide sequencing, identifying unknown proteins and determining post-transnational modifications. Samples can be extracted from such sources as gels, PVDF membranes, TLC plates, and/or Biacore chips. The fulfillment of the specific aims described in detail in the twelve research proposals would be facilitated by the addiction of a Voyager-DE MALDI-TOF