Symphony Multiplex Peptide Synthesizer w/ VISION Workstn

带 VISION Workstn 的 Symphony 多重肽合成器

• 批准号: 6733418
• 负责人: KENNETH G MANN
• 金额: $ 17.91万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6733418
• 关键词: biomedical equipment purchase; combinatorial chemistry; high performance liquid chromatography; peptide chemical synthesis; protein purification

DESCRIPTION (provided by applicant): This request is for an automated peptide synthesizer with a combinatorial reactor and a dedicated preparative HPLC. We have chosen the Symphony Multiplex from Protein Technologies Inc., as the peptide synthesizer and the VISION Workstation from Applied Biosystems as the preparative HPLC system. The Biochemistry Department at the University of Vermont has maintained a peptide synthesis capacity since 1984. The most recent instrument, a Chemtech model 90, has operated since 1993 is at the end of its lifetime and has limited capacity to support multiple investigators. There are multiple researchers at the University of Vermont with the need for peptides and with this award we intend to establish a core facility that would make peptides primarily for core users. We have requested the Symphony Multiplex because it has the capabilities to meet the needs of the research activities of multiple NIH funded research programs which include the development of reagents for antibody development, the evaluation of super-molecular organization of protein-ligand complexes, cellular regulation studies including protein-nucleic acid interactions, blood coagulation mechanism, muscle function, signal transduction, antigen-antibody interactions, amino acid libraries, and novel synthetic enzymes. The peptide synthesis system will be incorporated into the Department of Biochemistry's analytical facility in the College of Medicine. Financial support will be provided by the Department of Biochemistry, the College of Medicine, the Vermont Cancer Center, and by user fees. The principle investigator, Dr. Kenneth Mann and co-principle investigator Dr. Robert Hondal have used peptide synthesis extensively for antibody development, studies of protein interaction and protein semi-synthesis. This instrument will be under the primary control of Dr. Hondal who will be assisted by a technical staff with expertise in the use of the protein synthesizer and other analytical tools in the facility. In addition, this instrument facility will be incorporated into the training enterprise for graduate students in Biochemistry, Chemistry and other departments at the University of Vermont. The preparative HPLC system will be used by the facility to purify peptides and other biomolecules. There is currently no preparative HPLC system on the campus of the University of Vermont and this system is badly needed for many investigators.

描述（由申请人提供）：本申请要求提供一种具有组合反应器和专用制备型HPLC的自动化肽合成仪。我们选择了来自Protein Technologies Inc.的Symphony Multiplex，作为肽合成仪，来自Applied Biosystems的VISION Workstation作为制备型HPLC系统。佛蒙特大学生物化学系自1984年以来一直保持着肽合成能力。自1993年以来使用的最新仪器是Chemtech 90型仪器，其使用寿命已接近尾声，支持多名调查员的能力有限。佛蒙特大学有多名研究人员需要肽，有了这个奖项，我们打算建立一个核心设施，主要为核心用户生产肽。我们要求Symphony Multiplex，因为它能够满足多个NIH资助研究项目的研究活动需求，包括抗体开发试剂的开发，蛋白质-配体复合物超分子组织的评价，细胞调节研究，包括蛋白质-核酸相互作用，凝血机制，肌肉功能，信号转导，抗原-抗体相互作用，氨基酸文库和新型合成酶。肽合成系统将被纳入医学院生物化学系的分析设施。财政支持将由生物化学系，医学院，佛蒙特州癌症中心和用户费用提供。主要研究者Kenneth Mann博士和共同主要研究者Robert Hondal博士已将肽合成广泛用于抗体开发，蛋白质相互作用和蛋白质半合成的研究。该仪器将由Hondal博士主要控制，Hondal博士将得到一名技术人员的协助，该技术人员具有使用该设施中蛋白质合成仪和其他分析工具的专门知识。此外，该仪器设施将纳入佛蒙特大学生物化学、化学和其他系研究生的培训企业。该设施将使用制备型HPLC系统来纯化肽和其他生物分子。目前佛蒙特大学校园内没有制备型HPLC系统，许多研究人员迫切需要该系统。