Symphony Multiplex Peptide Synthesizer w/ VISION Workstn

描述（由申请人提供）：本申请是一个带有组合反应器和专用制备高效液相色谱的自动肽合成仪。我们选择了Protein Technologies Inc.的Symphony Multiplex作为多肽合成器，并选择了Applied Biosystems的VISION工作站作为制备型HPLC系统。佛蒙特大学生物化学系自1984年以来一直保持着肽合成能力。最新的仪器是Chemtech型号90，自1993年以来一直在使用，目前已经到了使用寿命的尽头，支持多名研究人员的能力有限。佛蒙特大学有多名研究人员需要多肽，我们打算通过这个奖项建立一个核心设施，主要为核心用户制造多肽。我们之所以要求Symphony Multiplex，是因为它有能力满足多个NIH资助的研究项目的研究活动的需要，这些项目包括抗体开发试剂的开发，蛋白质配体复合物的超分子组织的评估，细胞调节研究，包括蛋白质-核酸相互作用，血液凝固机制，肌肉功能，信号转导，抗原-抗体相互作用，氨基酸文库，以及新的合成酶。多肽合成系统将并入医学院生物化学系的分析设备。财政支持将由生物化学系、医学院、佛蒙特癌症中心和用户费用提供。首席研究员Kenneth Mann博士和联合首席研究员Robert Hondal博士广泛地将肽合成用于抗体开发、蛋白质相互作用和蛋白质半合成的研究。该仪器将由Hondal博士主要控制，他将得到一名在使用该设施中的蛋白质合成器和其他分析工具方面具有专业知识的技术人员的协助。此外，该仪器设施将纳入佛蒙特大学生物化学、化学等专业研究生培训项目。制备型高效液相色谱系统将被该设施用于纯化多肽和其他生物分子。目前，佛蒙特大学校园内还没有制备型高效液相色谱系统，这是许多研究者急需的。

DESCRIPTION (provided by applicant): This request is for an automated peptide synthesizer with a combinatorial reactor and a dedicated preparative HPLC. We have chosen the Symphony Multiplex from Protein Technologies Inc., as the peptide synthesizer and the VISION Workstation from Applied Biosystems as the preparative HPLC system. The Biochemistry Department at the University of Vermont has maintained a peptide synthesis capacity since 1984. The most recent instrument, a Chemtech model 90, has operated since 1993 is at the end of its lifetime and has limited capacity to support multiple investigators. There are multiple researchers at the University of Vermont with the need for peptides and with this award we intend to establish a core facility that would make peptides primarily for core users. We have requested the Symphony Multiplex because it has the capabilities to meet the needs of the research activities of multiple NIH funded research programs which include the development of reagents for antibody development, the evaluation of super-molecular organization of protein-ligand complexes, cellular regulation studies including protein-nucleic acid interactions, blood coagulation mechanism, muscle function, signal transduction, antigen-antibody interactions, amino acid libraries, and novel synthetic enzymes. The peptide synthesis system will be incorporated into the Department of Biochemistry's analytical facility in the College of Medicine. Financial support will be provided by the Department of Biochemistry, the College of Medicine, the Vermont Cancer Center, and by user fees. The principle investigator, Dr. Kenneth Mann and co-principle investigator Dr. Robert Hondal have used peptide synthesis extensively for antibody development, studies of protein interaction and protein semi-synthesis. This instrument will be under the primary control of Dr. Hondal who will be assisted by a technical staff with expertise in the use of the protein synthesizer and other analytical tools in the facility. In addition, this instrument facility will be incorporated into the training enterprise for graduate students in Biochemistry, Chemistry and other departments at the University of Vermont. The preparative HPLC system will be used by the facility to purify peptides and other biomolecules. There is currently no preparative HPLC system on the campus of the University of Vermont and this system is badly needed for many investigators.