STUDIES OF THE ACTIVATION OF PROTHROMBIN IN WHOLE HUMAN BLOOD

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 子项目：a）凝血酶原的活化 B）研究人全血中凝血酶原的活化 c）出血时间伤口的血液凝固研究 进行这些研究以通过静脉切开术抽取（研究a和B）或简化模型（研究c）评价人全血中凝血酶原活化的关系。 这些技术正在几项不同的研究中使用。 在子项目a下，研究了组织因子诱导的出血或凝血异常（包括各种蛋白质缺乏）患者的凝血。 分析凝血酶生成及其促凝血底物的活化，以生成这些止血疾病的特征性凝血特征。 在研究B中，对个体进行6个月的研究，并在20分钟的设定终点评价其最终凝血酶生成。 这使我们能够生成可能具有诊断意义的个体化凝血谱。 研究c使用Simplate器械跟踪出血时间伤口中的凝血酶生成和血凝块形成。 从而使我们能够研究血管成分存在时的凝血。 还测试受试者的完整代谢组、脂质谱、血红蛋白图、protime、INR、活化部分凝血活酶时间、纤维蛋白原、抗凝血酶III、因子II、V、VII、IX和X。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Subprojects: a) Activation of Prothrombin b) Studies of the Activation of Prothrombin in Whole Human Blood c) Studies of Blood Coagulation from Bleeding Time Wounds These studies are conducted to evaluate the relationship of prothrombin activation in whole human blood either through a phlebotomy draw (study a and b) or a Simplate model (study c). These techniques are being utilized in several different studies. Under subproject a, the tissue factor induced clotting of patients with bleeding or clotting abnormalities, including various protein deficiencies, are studied. Thrombin generation and the activation of its procoagulant substrates are analyzed to generate characteristic coagulation profiles for these hemostatic disorders. In study b, individuals are studied over a 6 month period and evaluated for their final thrombin generation at a set end point of 20 minutes. This allows us to generate individualized coagulation profiles that might have diagnostic implications. Study c, utilizes the Simplate device to follow thrombin generation and clot formation through a bleeding time wound. Thus allowing us to study coagulation with the vascular component present. Subjects are also tested for a complete metabolic panel, lipid profile, hemagram, protime, INR, activated partial thromboplastin time, fibrinogen, antithrombin III, factors II, V, VII, IX, and X.