STUDIES OF THE ACTIVATION OF PROTHROMBIN IN WHOLE HUMAN BLOOD

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分项目：a)激活凝血酶原 2)人全血中凝血酶原激活作用的研究 C)出血时间创面的血液凝固研究 这些研究是通过抽血(研究a和研究b)或单纯模型(研究c)来评估全血中凝血酶原激活的关系。这些技术正被用于几项不同的研究。在子项目a中，研究了组织因子诱导的出血或凝血异常患者的凝血，包括各种蛋白质缺乏。分析凝血酶的生成及其促凝血剂底物的激活，以生成这些止血疾病的特征凝血曲线。在研究b中，对个体进行了为期6个月的研究，并在设定的终点20分钟评估他们的最终凝血酶生成。这使我们能够生成可能具有诊断意义的个性化凝血曲线。研究c，利用Simplate设备通过出血时间伤口跟踪凝血酶的生成和凝块的形成。从而使我们能够研究凝血与血管成分的存在。 受试者还接受了完整的代谢组、血脂谱、血象、ProTime、INR、激活的部分凝血活酶时间、纤维蛋白原、抗凝血酶III、因子II、V、VII、IX和X的测试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Subprojects: a) Activation of Prothrombin b) Studies of the Activation of Prothrombin in Whole Human Blood c) Studies of Blood Coagulation from Bleeding Time Wounds These studies are conducted to evaluate the relationship of prothrombin activation in whole human blood either through a phlebotomy draw (study a and b) or a Simplate model (study c). These techniques are being utilized in several different studies. Under subproject a, the tissue factor induced clotting of patients with bleeding or clotting abnormalities, including various protein deficiencies, are studied. Thrombin generation and the activation of its procoagulant substrates are analyzed to generate characteristic coagulation profiles for these hemostatic disorders. In study b, individuals are studied over a 6 month period and evaluated for their final thrombin generation at a set end point of 20 minutes. This allows us to generate individualized coagulation profiles that might have diagnostic implications. Study c, utilizes the Simplate device to follow thrombin generation and clot formation through a bleeding time wound. Thus allowing us to study coagulation with the vascular component present. Subjects are also tested for a complete metabolic panel, lipid profile, hemagram, protime, INR, activated partial thromboplastin time, fibrinogen, antithrombin III, factors II, V, VII, IX, and X.