STUDIES OF THE ACTIVATION OF PROTHROMBIN IN WHOLE HUMAN BLOOD

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 子项目：a) 凝血酶原的激活 b) 人全血中凝血酶原激活的研究 c) 伤口出血时的血液凝固研究 这些研究旨在通过放血（研究 a 和 b）或 Simplate 模型（研究 c）来评估人类全血中凝血酶原激活的关系。 这些技术正在几项不同的研究中得到利用。 在子项目a下，研究了组织因子诱导出血或凝血异常（包括各种蛋白质缺乏）患者的凝血。 分析凝血酶的产生及其促凝血底物的激活，以生成这些止血疾病的特征性凝血曲线。 在研究 b 中，对个体进行了为期 6 个月的研究，并在设定的 20 分钟终点评估其最终凝血酶的生成。 这使我们能够生成可能具有诊断意义的个体化凝血曲线。 研究 c，利用 Simplate 装置跟踪出血时伤口的凝血酶生成和凝块形成。 从而使我们能够研究存在血管成分的凝血。 还对受试者进行完整的代谢组、血脂谱、血红蛋白、蛋白质、INR、活化部分凝血活酶时间、纤维蛋白原、抗凝血酶 III、因子 II、V、VII、IX 和 X 的测试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Subprojects: a) Activation of Prothrombin b) Studies of the Activation of Prothrombin in Whole Human Blood c) Studies of Blood Coagulation from Bleeding Time Wounds These studies are conducted to evaluate the relationship of prothrombin activation in whole human blood either through a phlebotomy draw (study a and b) or a Simplate model (study c). These techniques are being utilized in several different studies. Under subproject a, the tissue factor induced clotting of patients with bleeding or clotting abnormalities, including various protein deficiencies, are studied. Thrombin generation and the activation of its procoagulant substrates are analyzed to generate characteristic coagulation profiles for these hemostatic disorders. In study b, individuals are studied over a 6 month period and evaluated for their final thrombin generation at a set end point of 20 minutes. This allows us to generate individualized coagulation profiles that might have diagnostic implications. Study c, utilizes the Simplate device to follow thrombin generation and clot formation through a bleeding time wound. Thus allowing us to study coagulation with the vascular component present. Subjects are also tested for a complete metabolic panel, lipid profile, hemagram, protime, INR, activated partial thromboplastin time, fibrinogen, antithrombin III, factors II, V, VII, IX, and X.