STUDIES OF THE ACTIVATION OF PROTHROMBIN IN WHOLE HUMAN BLOOD

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Subprojects: a) Activation of Prothrombin b) Studies of the Activation of Prothrombin in Whole Human Blood c) Studies of Blood Coagulation from Bleeding Time Wounds These studies are conducted to evaluate the relationship of prothrombin activation in whole human blood either through a phlebotomy draw (study a and b) or a Simplate model (study c). These techniques are being utilized in several different studies. Under subproject a, the tissue factor induced clotting of patients with bleeding or clotting abnormalities, including various protein deficiencies, are studied. Thrombin generation and the activation of its procoagulant substrates are analyzed to generate characteristic coagulation profiles for these hemostatic disorders. In study b, individuals are studied over a 6 month period and evaluated for their final thrombin generation at a set end point of 20 minutes. This allows us to generate individualized coagulation profiles that might have diagnostic implications. Study c, utilizes the Simplate device to follow thrombin generation and clot formation through a bleeding time wound. Thus allowing us to study coagulation with the vascular component present. Subjects are also tested for a complete metabolic panel, lipid profile, hemagram, protime, INR, activated partial thromboplastin time, fibrinogen, antithrombin III, factors II, V, VII, IX, and X.