BIOROBOT 3000

生物机器人3000

• 批准号: 6292232
• 负责人: Neil R. Hackett
• 金额: $ 12.96万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6292232
• 关键词: biomedical equipment; biomedical equipment purchase; microarray technology; nucleic acid sequence

DESCRIPTION: (Investigator?s Abstract): This application requests funds to purchase a liquid handling and nucleic acid preparation robot that represents part of a coordinated plan to build a superior Core Facilities for Gene Therapy and Gene Expression analysis at The Weill Medical College of Cornell University. These shared facilities will provide Cornell and affiliated faculty with the technology to analyze gene expression by array methods and the technology of gene therapy from basic research to clinical trials. Based on time utilization studies of the initial users of the Gene Therapy Core Facility, we have determined that routine repetitive operations of molecular biology use an inordinate amount of the time of skilled technicians and are prone to error when done in large numbers. The BioRobot 3000 represents a flexible and versatile system that can readily be used by a number of researchers for sample preparation with high throughput and reproducibility. A group of five major or and three minor investigators with extensive research support, primarily from NIH sources, have been identified as the initial users of the gene expression gene therapy cores and represent the users of the equipment. Among the intended applications are routine preparation of plasmid DNA, genomic DNA and RNA preparation from in vitro cell culture and in vivo studies of gene transfer, and PCR reaction setup. In the context of the gene expression core, the plasmid preparation and post-PCR clean up applications will be used to provide raw material for spotting arrays. In the overall organizational structure and facilities of the Gene Therapy Core Facility provide the location, permanent management and oversight mechanisms that ensures the optimal use of the instrument.

描述：（研究者摘要）：本申请要求资金 购买代表液体处理和核酸制备的机器人 建设卓越的基因治疗核心设施的协调计划的一部分 康奈尔威尔医学院的基因表达分析 大学。 These shared facilities will provide Cornell and affiliated faculty 通过阵列方法分析基因表达的技术 基因治疗技术从基础研究到临床试验。基于 基因治疗核心初始用户的时间利用研究 设施，我们已经确定分子的常规重复操作 生物学占用了熟练技术人员大量的时间，并且 大量操作时容易出错。 BioRobot 3000 代表了 灵活且多功能的系统，可以很容易地被许多人使用 研究人员进行高通量和可重复性的样品制备。一个 由五名主要或三名次要研究人员组成的小组，进行广泛的研究 主要来自 NIH 来源的支持已被确定为初始用户 基因表达基因治疗核心的成员并代表该基因的用户 设备。预期应用包括质粒的常规制备 从体外细胞培养物和体内制备 DNA、基因组 DNA 和 RNA 基因转移和PCR反应设置的研究。在基因背景下 表达核心、质粒制备和 PCR 后净化应用 将用于为点样阵列提供原材料。在整体上 基因治疗核心设施的组织结构和设施 提供地点、永久管理和监督机制 确保仪器的最佳使用。