CORE--VECTOR 1--ADENOVIRUS/ADENO ASSOCIATED VIRUS HERPESVIRUS LIPOSOME

核心--载体1--腺病毒/腺相关病毒疱疹病毒脂质体

• 批准号: 6501118
• 负责人: Neil R. Hackett
• 金额: $ 12.63万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6501118
• 关键词: Adenoviridae; adeno associated virus group; biomedical facility; herpes simplex virus 1; liposomes; microorganism culture; transfection /expression vector

Core A (Vector 1) will provide assistance in the design, construction and struCtural verification of gene therapy vectors based on adenovirus (Ad), adeno-associated virus (AAV), herpes simplex virus I (HSV). The major role of the core is to provide knowledge and biological materials to the investigators so they may focus on the biological aspects of their projects rather than the technology of gene therapy. For this reason the core will fulfill the following roles: Perform large scale vector preparations for the participating projects (target 50 preparations /year) in accordance with the core usage allocations and resources available; Create new gene therapy vectors by importing improvements in the technology and / or new transgenes that are important for the participating projects (target 18 / year); Provide Standard Operating Procedures for all phases in the construction of all three types of viral vector; Education in construction of vectors through tutorials with senior core scientists and shadowing core technicians; Supply verified samples of biological materials for the vector construction including plasmids, cell lines and starting vectors, and maintain a database describing those materials.

核心A（矢量1）将在设计中提供帮助， 基因治疗载体的构建和结构验证 基于腺病毒（Ad）、腺相关病毒（AAV）、疱疹病毒 单纯病毒I（HSV）。核心的主要作用是提供 知识和生物材料的调查，使他们可以 专注于项目的生物学方面，而不是 基因治疗技术。因此，核心将满足 执行大规模的矢量准备工作， 参与项目（目标是每年50项筹备工作） 核心使用分配和可用资源;创建新的 基因治疗载体通过引进技术的改进， /或对参与者重要的新转基因 项目（目标18个/年）;提供标准作业程序 对于所有三种类型的病毒构建的所有阶段， 矢量;通过教程进行矢量构建教育， 高级核心科学家和跟踪核心技术人员;供应 用于载体构建的经验证的生物材料样品 包括质粒、细胞系和起始载体， 一个描述这些材料的数据库