CORE--VECTOR

核心--向量

• 批准号: 6318393
• 负责人: Neil R. Hackett
• 金额: $ 25.26万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6318393
• 关键词: Adenoviridae; biomedical facility; biotechnology; technology /technique development; transfection /expression vector

Core A (Vector) will provide assistance in the design, construction and structural verification of gene therapy vectors based on adenovirus (Ad). The major role of the core is to provide service, knowledge and biological materials to the investigators so they may focus on the biological aspects of their projects rather than the technology of Ad vector construction. For this reason the core will fulfill the following roles for all four of the projects in the program: Perform large scale vector preparations for the participating projects (target 50 preparations/year) in accordance with the core usage allocations and resources available; Create new gene therapy vectors by importing improvements in the technology and/or new transgenes and capsid modifications that are important for the participating projects (target 18/year); Provide Standard Operating Procedures for all phases in the construction of all Ad vectors; Education of project scientists in construction of vectors through tutorials with senior core scientists and shadowing core technicians; Supply verified samples of biological samples for the vector construction including plasmids, cell lines and starting vectors, and maintain a database describing those materials.

核心A（矢量）将提供设计，施工和 基于腺病毒基因治疗载体的结构验证 （广告）。核心的主要作用是提供服务、知识和 生物材料的调查，使他们可以专注于 他们的项目的生物方面，而不是广告技术 载体构建因此，核心将满足以下要求 项目中所有四个项目的角色： 参与项目的媒介准备工作（目标50 （a）按照核心用途分配， 可利用的资源;通过导入新的基因治疗载体 技术的改进和/或新的转基因和衣壳 对参与项目重要的修改（目标 18个/年）;为所有阶段提供标准操作程序 所有广告载体的构建; 通过与资深核心科学家的教程构建载体 并跟踪核心技术人员; 用于载体构建的样品，包括质粒、细胞系和 启动矢量，并维护描述这些材料的数据库。