STORM 860 PHOSPHORIMAGER

这个申请购买磷光成像仪的申请代表了在康奈尔大学威尔医学院建立一个高级基因治疗设施的协调计划的一部分。这个共享设施将为康奈尔大学及其附属教师提供从基础研究到临床试验的所有基因治疗技术。为了促进高质量的基础研究，分子核心负责提供分析方法和仪器，以便对生物样品进行详细的分子分析。基因治疗的成功关键取决于对基因转移效率的客观定量评估和对治疗组织状态的分析。传统的x射线和照相胶片在分子生物学中的应用限制了定量分析。这里要求的多功能磷成像仪将使几种定量方法能够使用荧光，化学荧光和放射性方法分析分子数据。一个由12名研究人员组成的小组得到了广泛的研究支持，主要来自美国国立卫生研究院，已被确定为基因治疗设备的初始用户，并代表设备的用户。预期的应用包括对组织中治疗性和内源性基因表达的定量分析，以及使用基因阵列对基因表达和基因治疗效果进行全面调查。在基因治疗设施的整体组织结构和设施中，提供位置和永久管理和监督机制，以确保仪器的最佳使用。

This application for funds to purchase a phosphorimager represents part of a coordinated plan to build a superior Gene Therapy Facility at The Weill Medical College of Cornell University. This shared facility will provide Cornell and affiliated faculty with access to all the technology to perform gene therapy from basic research to clinical trials. To promote high quality basic research, the molecular core is charged with providing analytical methods and instruments that allow detailed molecular analyses of biological samples. Success in gene therapy is critically dependent on objective quantitative assessment of the efficiency of gene transfer and analysis of the status of treated of tissues. The traditional use of X-ray and photographic film in molecular biology has limited quantitative analyses. The multi-functional phosphorimager requested here will enable several quantitative approaches to the analysis of molecular data using fluorescent, chemifluorescent and radioactive approaches. A group of twelve investigators with extensive research support, primarily from NIH sources, have been identified as the initial users of the Gene Therapy Facility and represent the users of the equipment. Among the intended applications are quantitative assays of therapeutic and endogenous gene expression in tissues and use of gene arrays to give comprehensive surveys of gene expression and the effect of gene therapy. In the overall organizational structure and facilities of the Gene Therapy Facility provide the location and permanent management and oversight mechanisms that ensures the optimal use of the instrument.