FUNCTIONAL ANALYSIS OF A FAMILY OF CELL WALL ASSOCIATED RECEPTOR KINASES

细胞壁相关受体激酶家族的功能分析

• 批准号: 6395901
• 负责人: Zheng-Hui HE
• 金额: $ 12.7万
• 依托单位: SAN FRANCISCO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6395901
• 关键词: Arabidopsis; biological signal transduction; cell adhesion molecules; cell cell interaction; cell wall; cytoplasm; extracellular matrix; fluorescence microscopy; gene expression; gene interaction; gene targeting; genetic transcription; green fluorescent proteins; nucleic acid probes; nucleic acid sequence; phenotype; plant growth /development; polymerase chain reaction; protein kinase; protein structure function; southern blotting

In multi-cellular organisms the interactions between extracellular and intracellular compartments play an important role in controlling many developmental decisions. In animal cells there has been solid evidence that extracellular matrix (ECM) molecules are constituents of selective cell-cell recognition and cell-ECM adhesion processes and are important in regulation of developmental processes such as cellular polarity, differentiation, cell division, cell death and cell migration. This proposal concerns a family of five ECM-associated receptor-like protein kinases, Waks (Wall Associated Kinase), which appear to be adhesion receptors that exchange signals between the extracellular and intracellular compartments of Arabidopsis. Waks have a transmembrane domain that separates an amino-terminal, extracytoplasmic domain that have EGF (Epidermal Growth Factor) repeats and limited sequence identities to either the tenascin superfamily, or the neurexins. The carboxyl region of all the Waks encode a cytoplasmic serine/threonine protein kinase. Waks are localized in the plasma membrane. Moreover, the Wak1 kinase domain interacts with a cytoplasmic type 2C protein phosphatase. Both an insertional mutation and a dominant negative mutation of Wak1 have been isolated and found to be lethal, indicating that the protein is essential. This proposal describes experiments to dissect the signaling pathways associated with Waks and to determine the role Waks have in signal transduction between the plant ECM and the cytoplasm. The analysis of the Wak family of proteins may lead to a description of a new signal transduction mechanism in plant cells, and perhaps toward general principals of how eukaryotic cells perceive architectural changes in their extracellular matrix and environment.

在多细胞生物体中，细胞外和细胞间的相互作用 细胞内的隔间在控制许多 发展决策。在动物细胞中，有确凿的证据表明 细胞外基质(ECM)分子是选择性 细胞-细胞识别和细胞-ECM黏附过程在 对发育过程的调节，如细胞的极性， 分化、细胞分裂、细胞死亡和细胞迁移。这 该提案涉及一个五人家族的ECM相关受体样蛋白 看似粘连的蛋白激酶、WAKS(壁联蛋白激酶) 在细胞外和细胞外之间交换信号的受体 拟南芥的胞内分室。WAKS有一个跨膜 分离氨基末端的结构域，胞外结构域具有 EGF(表皮生长因子)重复序列和有限序列同一性 要么是Tenascin超家族，要么是Neurexins。的羧基区域 所有的WAK都编码一种细胞质丝氨酸/苏氨酸蛋白激酶。瓦克斯 定位于质膜。此外，Wak1激酶结构域 与细胞质中的2C蛋白磷酸酶相互作用。两者都是 Wak1的插入突变和显性负突变 被分离并被发现是致命的，表明这种蛋白质是必不可少的。 这项建议描述了剖析信号通路的实验。 与WAKS相关联，并确定WAKS在信号中的角色 植物细胞外基质与细胞质之间的转导。对中国传统文化的分析与思考 WAK蛋白家族可能导致对一种新信号的描述 在植物细胞中的转导机制，并可能通向一般 真核细胞如何感知其结构变化的原理 细胞外基质和环境。