FUNCTIONAL ANALYSIS OF A FAMILY OF CELL WALL ASSOCIATED RECEPTOR KINASES

细胞壁相关受体激酶家族的功能分析

• 批准号: 6107759
• 负责人: Zheng-Hui HE
• 金额: $ 12.7万
• 依托单位: SAN FRANCISCO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6107759
• 关键词: Arabidopsis; biological signal transduction; cell adhesion molecules; cell cell interaction; cell wall; cytoplasm; extracellular matrix; fluorescence microscopy; gene expression; gene interaction; gene targeting; genetic transcription; green fluorescent proteins; nucleic acid probes; nucleic acid sequence; phenotype; plant growth /development; polymerase chain reaction; protein kinase; protein structure function; southern blotting

In multi-cellular organisms the interactions between extracellular and intracellular compartments play an important role in controlling many developmental decisions. In animal cells there has been solid evidence that extracellular matrix (ECM) molecules are constituents of selective cell-cell recognition and cell-ECM adhesion processes and are important in regulation of developmental processes such as cellular polarity, differentiation, cell division, cell death and cell migration. This proposal concerns a family of five ECM-associated receptor-like protein kinases, Waks (Wall Associated Kinase), which appear to be adhesion receptors that exchange signals between the extracellular and intracellular compartments of Arabidopsis. Waks have a transmembrane domain that separates an amino-terminal, extracytoplasmic domain that have EGF (Epidermal Growth Factor) repeats and limited sequence identities to either the tenascin superfamily, or the neurexins. The carboxyl region of all the Waks encode a cytoplasmic serine/threonine protein kinase. Waks are localized in the plasma membrane. Moreover, the Wak1 kinase domain interacts with a cytoplasmic type 2C protein phosphatase. Both an insertional mutation and a dominant negative mutation of Wak1 have been isolated and found to be lethal, indicating that the protein is essential. This proposal describes experiments to dissect the signaling pathways associated with Waks and to determine the role Waks have in signal transduction between the plant ECM and the cytoplasm. The analysis of the Wak family of proteins may lead to a description of a new signal transduction mechanism in plant cells, and perhaps toward general principals of how eukaryotic cells perceive architectural changes in their extracellular matrix and environment.

在多细胞生物中，细胞外和细胞之间的相互作用 细胞内区室在控制许多 发展决策。在动物细胞中已有确凿的证据 细胞外基质（ECM）分子是选择性的成分 细胞与细胞的识别和细胞与 ECM 的粘附过程在 发育过程的调节，例如细胞极性， 分化、细胞分裂、细胞死亡和细胞迁移。这 提案涉及五个 ECM 相关受体样蛋白家族 激酶，Waks（壁相关激酶），似乎与粘附有关 细胞外和细胞之间交换信号的受体 拟南芥的细胞内区室。 Waks具有跨膜 分隔氨基末端、胞质外结构域的结构域 EGF（表皮生长因子）重复序列和有限序列同一性 腱蛋白超家族或神经毒素。羧基区 所有 Waks 都编码细胞质丝氨酸/苏氨酸蛋白激酶。瓦克斯 定位于质膜。此外，Wak1 激酶结构域 与细胞质 2C 型蛋白磷酸酶相互作用。两者都是 Wak1 的插入突变和显性失活突变 分离并发现具有致命性，表明该蛋白质是必需的。 该提案描述了剖析信号通路的实验 与 Waks 相关并确定 Waks 在信号中的作用 植物 ECM 和细胞质之间的转导。的分析 Wak 蛋白家族可能会导致对新信号的描述 植物细胞中的转导机制，并可能走向普遍 真核细胞如何感知其结构变化的原理 细胞外基质和环境。