Role of a Cell Wall-Associated Kinase in Zinc Sensing

细胞壁相关激酶在锌传感中的作用

• 批准号: 7751322
• 负责人: Zheng-Hui HE
• 金额: $ 19.64万
• 依托单位: SAN FRANCISCO STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7751322
• 关键词: Affect; Animals; Arabidopsis; Biochemical Genetics; Bioinformatics; Blood; Cell Wall; Cell membrane; Cells; Communication; Data; Diet; Dietary Zinc; Dissection; Edible Plants; Environment; Extracellular Matrix; Family; Family member; Foundations; Genes; Genetic; Genetic Screening; Goals; Growth; Health; Human; Knock-out; Knowledge; Left; Link; Mediating; Membrane; Micronutrients; Minerals; Molecular; Molecular Genetics; Monitor; Mouse-ear Cress; Mutation; Organ; Organism; Phosphotransferases; Plant Model; Plant Roots; Plants; Play; Production; Public Health; Publishing; Regulation; Reproduction; Research; Role; San Francisco; Screening procedure; Signal Pathway; Signal Transduction; Solid; Source; Testing; Tissues; Transgenic Organisms; Universities; Work; Wound Healing; Zinc; Zinc deficiency; base; experience; extracellular; improved; innovation; loss of function; member; mutant; nutrition; receptor; response; zinc-binding protein

multicellular organisms sense zinc and consequently regulate zinc transport is largely unknown. The main goal of our research is to understand how a family of cell wall-associated receptor kinases (WAK) and WAK-like (WAKL) kinases function in cell-cell and cell-environment communications. We have discovered that WAKL4, a WAK/WAKL family member, plays a critical role in Arabidopsis zinc sensing. Our objective in this application is to elucidate the mechanisms of how plants use WAKL4 to monitor extracellular zinc conditions and to regulate zinc transport. Our central hypothesis is that WAKL4, as a receptor kinase, is one of the key regulatory components in zinc signaling. We propose to test this hypothesis to identify key regulators in Arabidopsis zinc signaling. In Aim 1, we will test if zinc-induced tissue-specific WAKL4 expression plays a role in zinc sensing and zinc transport by identifying the actual components that control zinc-induced WAKL4 expression. A combination of biochemical, genetic and bioinformatic approaches will be used to identify and to determine the roles of the regulatory components. In Aim 2, we will use suppressor screening to identity other critical genes in the WAKL4- mediated zinc signaling. The genetic screening is based on the fact that in the absence of WAKL4, zinc transporter genes fail to respond to external zinc depletion. We will identify mutants whose zinc transporter genes, in absence of WAKL4, remain responding to zinc deficiency. WAKL4 is the first receptor kinase suggested as having a role in mineral sensing and regulation. Our proposed studies are expected to provide critical information in understanding how Arabidopsis uses receptor kinases to sense and respond to zinc, and to significantly advance the understanding of how WAK/WAKL members communicate between the extracellular and intracellular compartments. Relevance to Public Health: Zinc is an essential micronutrient for all organisms and its deficiency is a well-known human-health problem. Our contribution here is expected to be a detailed understanding of how an extracellular matrix-linked receptor kinase plays a role in plant zinc sensing and transport. This contribution is expected to provide the knowledge needed to significantly improve edible plants as better dietary zinc sources for both animals and humans.

多细胞生物感知锌并因此调节锌的运输在很大程度上是未知的。这 我们研究的主要目标是了解细胞壁相关受体激酶 (WAK) 家族如何 和 WAK 样 (WAKL) 激酶在细胞-细胞和细胞-环境通讯中发挥作用。我们有 发现 WAKL4（WAK/WAKL 家族成员）在拟南芥锌感应中发挥着关键作用。 我们在此应用中的目标是阐明植物如何使用 WAKL4 来监测的机制 细胞外锌条件并调节锌转运。我们的中心假设是 WAKL4，作为 受体激酶是锌信号传导的关键调节成分之一。我们建议对此进行测试 确定拟南芥锌信号传导关键调节因子的假设。在目标 1 中，我们将测试锌是否诱导 组织特异性 WAKL4 表达通过识别实际的锌感应和锌转运来发挥作用 控制锌诱导的 WAKL4 表达的成分。生物化学、遗传和 将使用生物信息方法来识别和确定监管的作用 成分。在目标 2 中，我们将使用抑制子筛选来识别 WAKL4- 中的其他关键基因 介导的锌信号传导。基因筛查基于以下事实：在缺乏 WAKL4 的情况下，锌 转运蛋白基因无法对外部锌耗尽做出反应。我们将鉴定出含有锌的突变体 在缺少 WAKL4 的情况下，转运蛋白基因仍然对锌缺乏做出反应。 WAKL4是第一个 受体激酶被认为在矿物质传感和调节中发挥作用。我们提出的研究是 预计将为了解拟南芥如何使用受体激酶来感知提供关键信息 并对锌做出反应，并显着增进对 WAK/WAKL 成员如何 细胞外和细胞内区室之间的通讯。 与公共健康的相关性：锌是所有生物体必需的微量营养素，其缺乏是一个严重的问题。 众所周知的人类健康问题。我们在这里的贡献预计是详细了解 细胞外基质连接受体激酶如何在植物锌传感和运输中发挥作用。这 预计贡献将提供显着改善食用植物所需的知识 动物和人类的膳食锌来源。