Core--Mass spectrometry

这个质谱学核心的广泛目标是提供高端质谱学技术，以支持这项超级基金基础研究计划中的生物医学和非生物医学项目，并开发和应用一种新的、高度特异和灵敏的质谱学技术来检测和量化环境化合物及其代谢物。这些化合物包括四氯乙烯、1，1，2-三氯乙烯、顺-1，2-二氯乙烯、氯乙烯、氯乙醛和氯仿。这一新仪器的开发将需要建造一台气相色谱仪电子单色谱仪飞行时间质谱仪(GC-EM-TOF-MS)来分析负离子，利用EM产生单能电离电子束，并结合脉冲提取。为了支持其他生物医学和非生物医学项目的这一应用，我们将开发和应用质谱学方法来分析蛋白质和碳氢化合物溶剂代谢物之间形成的加合物。我们将开发与2，5-己二酮及其类似物3-甲基-2，5-己二酮、3，4-二甲基-2，5-己二酮、3-4-二乙基-2，5-己二酮、2，9-二甲基-4，7、癸二酮和1，2-二乙酰苯反应产生的蛋白质和多肽的质谱分析方法。将通过氢-氢交换(H/D)交换和平衡热变性研究结合电喷雾质谱来评估伽马-二酮烃加合物对神经丝蛋白质结构热稳定性的影响。碳氢加合物对神经丝蛋白亚基构象的影响将通过动力学H/D交换实验和电喷雾光谱分析来确定。

The broad objective of this Mass Spectrometry Core is to provide high- end mass spectroscopy techniques to support the biomedical and non- biomedical projects in this Superfund Basic Research Program and to develop and apply a new, highly specific and sensitive mass spectroscopy technique to detect and quantify environmental compounds and their metabolites. These compounds will include tetrachloroethene, 1,1,2- trichloroethene, cis-1,2-dichloroethene, vinyl chloride, chloroacetaldehyde and chloroform. The development of this new instrument will require the construction of a gas chromatograph electron monochromater-time-of flight-mass spectrometer (GC-EM-TOF-MS) to analyze negative ions using the EM for the production of monoenergetic beams of ionizing electrons in combination with pulsed extraction. To support the other biomedical and non-biomedical projects in this application, we will develop and apply mass spectrometric methods to analyze adducts formed between proteins and hydrocarbon solvent metabolites. We will develop mass spectrometric methods for analyzing protein and peptides resulting from reaction with 2,5-hexanedione, and analogs, 3-methyl-2,5-hexanedione, 3,4-dimethyl-2,5-hexanedione, 3-4- diethyl-2,5-hexanedione, 2,9-dimethyl-4,7, decanedione and 1,2- diacetylbenzene. The effects of gamma-diketone hydrocarbon adducts on the thermal stability of neurofilament protein structures will be a assessed by hydrogen-deuterium exchange (H/D) exchange and equilibrium thermal denaturation studies in conjunction with electrospray mass spectroscopy. The effects of hydrocarbon adducts on the conformation of neurofilament protein subunits will be determined by kinetic H/D exchange experiments, together with electrospray mas spectrometry.