HYBRID LC/MS/MS QUADRUPOLE TOF MASS SPECTROMETER

俄勒冈州立大学化学和环境健康科学中心(EHSC)向NCRR申请资金，以购买新的Q-T混合四极杆/正交飞行时间质谱仪和数据系统(MS/MS/DS)。需要这种仪器来填补俄亥俄州立大学现有能力中的空白，以解决质谱学实验室中持续存在的问题。俄亥俄州立大学质谱学实验室目前可用的仪器没有解决我们越来越多地遇到的许多蛋白质结构问题所需的综合灵敏度和分辨率。新仪器将支持的研究项目大致分为两个主要领域。这些是：1)通过分析2-D凝胶斑点酶鉴定蛋白质；2)对折叠中间体和由修饰和配基相互作用产生的构象状态进行氢/氢交换研究。此外，该仪器还将用于确定蛋白质修饰和磷酸化的位置、肽序列和海洋生物碱结构。主要用户拥有美国国立卫生研究院的资金，用于开展申请中所述的研究。虽然EHSC、部门资金和设施费用为质谱仪设施提供了支持，但没有建立机制来筹集足够的资金来购买主要设备。新仪器的次要作用将是为那些要求高分辨率、质量准确性和灵敏度的调查人员提供校园范围内的质谱学支持。俄勒冈州州立大学的质谱学设施也支持俄勒冈州其他主要学术机构的质谱学需求。

The Department of Chemistry and Environmental Health Sciences Center (EHSC) at Oregon State University requests funds from the NCRR to purchase a new Q-T of Hybrid Quadrupole/Orthogonal Time- of-Flight Mass Spectrometer and Data System (MS/MS/DS). This instrument is needed to fill a void in the existing capabilities at OSU to solve ongoing problems in the mass spectrometry laboratory. The instrumentation that is currently available in the OSU mass spectrometry laboratory does not have the combined sensitivity and resolving power necessary to solve many of the protein structural problems we are increasingly encountering. The research projects that will be supported by the new instrument fall generally into two major areas. These are 1) protein identification from analysis of 2-D gel spot digests and 2) hydrogen/deuterium exchange studies on folding intermediates and conformational states resulting from modifications and ligand interactions. In addition the instrument will be used for identifying sites of protein modifications and phosphorylation, peptide sequences, and marine alkaloid structures. The major users have NIH funding to carry out the studies described in the application. Although EHSC, departmental funds and facility charges have provided support for the mass spectrometry facility, there is no mechanism in place to raise sufficient funds to purchase major equipment. A secondary role of the new instrument will be to provide campus-wide mass spectrometry support for those investigators requiring high resolution, mass accuracy, and sensitivity. The OSU Mass Spectrometry Facility also supports the mass spectrometry needs of the other major Oregon academic institutions.