Core--Mass spectrometry

核心--质谱

• 批准号: 6577813
• 负责人: MAX L DEINZER
• 金额: $ 10.08万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6577813
• 关键词: biomedical facility; chlorine; chloroform; electron microscopy; environmental toxicology; ethylenes; gas chromatography mass spectrometry; halobiphenyl /halotriphenyl compound; hazardous substances; mass spectrometry; matrix assisted laser desorption ionization; trichloroethylene

The broad objective of this Mass Spectrometry Core is to provide high- end mass spectroscopy techniques to support the biomedical and non- biomedical projects in this Superfund Basic Research Program and to develop and apply a new, highly specific and sensitive mass spectroscopy technique to detect and quantify environmental compounds and their metabolites. These compounds will include tetrachloroethene, 1,1,2- trichloroethene, cis-1,2-dichloroethene, vinyl chloride, chloroacetaldehyde and chloroform. The development of this new instrument will require the construction of a gas chromatograph electron monochromater-time-of flight-mass spectrometer (GC-EM-TOF-MS) to analyze negative ions using the EM for the production of monoenergetic beams of ionizing electrons in combination with pulsed extraction. To support the other biomedical and non-biomedical projects in this application, we will develop and apply mass spectrometric methods to analyze adducts formed between proteins and hydrocarbon solvent metabolites. We will develop mass spectrometric methods for analyzing protein and peptides resulting from reaction with 2,5-hexanedione, and analogs, 3-methyl-2,5-hexanedione, 3,4-dimethyl-2,5-hexanedione, 3-4- diethyl-2,5-hexanedione, 2,9-dimethyl-4,7, decanedione and 1,2- diacetylbenzene. The effects of gamma-diketone hydrocarbon adducts on the thermal stability of neurofilament protein structures will be a assessed by hydrogen-deuterium exchange (H/D) exchange and equilibrium thermal denaturation studies in conjunction with electrospray mass spectroscopy. The effects of hydrocarbon adducts on the conformation of neurofilament protein subunits will be determined by kinetic H/D exchange experiments, together with electrospray mas spectrometry.

该质谱核心的广泛目标是提供高端质谱技术来支持超级基金基础研究计划中的生物医学和非生物医学项目，并开发和应用一种新的、高度特异性和灵敏的质谱技术来检测和量化环境化合物及其代谢物。这些化合物包括四氯乙烯、1,1,2-三氯乙烯、顺式1,2-二氯乙烯、氯乙烯、氯乙醛和氯仿。这种新仪器的开发需要建造气相色谱电子单色仪-飞行时间质谱仪 (GC-EM-TOF-MS)，以使用 EM 分析负离子，并结合脉冲提取产生电离电子的单能束。为了支持本应用中的其他生物医学和非生物医学项目，我们将开发并应用质谱方法来分析蛋白质和烃溶剂代谢物之间形成的加合物。我们将开发质谱方法来分析与2,5-己二酮及类似物、3-甲基-2,5-己二酮、3,4-二甲基-2,5-己二酮、3-4-二乙基-2,5-己二酮、2,9-二甲基-4,7、癸二酮和1,2-己二酮反应产生的蛋白质和肽。 二乙酰苯。 γ-二酮烃加合物对神经丝蛋白结构热稳定性的影响将通过氢-氘交换（H/D）交换和平衡热变性研究结合电喷雾质谱来评估。烃加合物对神经丝蛋白亚基构象的影响将通过动力学 H/D 交换实验以及电喷雾质谱法来确定。