MARKERS OF PROTEIN OXIDATION BY HYDROXYL RADICAL & REACTIVE NITROGEN SPECIES

羟基自由基氧化蛋白质的标志物

• 批准号: 6336806
• 负责人: CHRISTIAAN LEEUWENBURGH
• 金额: $ 0.6万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6336806
• 关键词: animal tissue; biological products; biomedical resource; carbohydrates; diabetes mellitus; endocrine gland /system; hormones; immunology; inflammation; lipids; spectrometry

Many lines of evidence implicate oxidative damage in aging. Possible pathways include reactions that modify aromatic amino acid residues on proteins. o-Tyrosine is a stable marker for oxidation of protein-bound phenylalanine by hydroxyl radical, whereas 3-nitrotyrosine is a marker for oxidation of protein-bound tyrosine by reactive nitrogen species. To test the hypothesis that proteins damaged by hydroxyl radical and reactive nitrogen accumulate with aging, we used stable isotope dilution gas chromatography-mass spectrometry (GC/MS) to measure levels of o-tyrosine and 3-nitrotyrosine in heart, skeletal muscle and liver from young adult (9 months) and old (24 month) female Long Evans/Wistar hybrid rats. We also measured these markers in young adult and old rats that received antioxidant supplements ((-tocopherol, (-carotene, butylated hydroxytoluene and ascorbic acid) from the age of 5 months. We found that aging did not significantly increase levels of protein-bound o-ty rosine or 3-nitrotyrosine in any of the tissues. Antioxidant supplementation had no effect on the levels of protein-bound o-tyrosine and 3-nitrotyrosine in either young or old animals. These observations indicate that the o-tyrosine and 3-nitrotyrosine do not increase significantly in heart, skeletal muscle and liver in old rats, suggesting that proteins damaged by hydroxyl radical and reactive nitrogen species do not accumulate in these tissues with advancing age.

许多证据表明氧化损伤与衰老有关。