SIGNAL TRANSDUCTION & TRANSCRIPTION PROTEIN (STAT)

信号转导

• 批准号: 6307614
• 负责人: JAMES E DARNELL
• 金额: $ 0.82万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6307614
• 关键词: animal tissue; biomedical resource; human tissue; proteins; spectrometry

Characterization of a C-terminal fragment of the recombinant STAT1 protein by MALDI-MS. Determination of the extent of alkylation as well as phosphorylation of the protein. Continuation of this project will include the determination of amino acid residues that are important for DNA binding using photocrosslinking and MALDI-MS peptide mapping. A paper describing this work has been published U. Vinkemeier, S.L. Cohen. I. Moarefi, B.T. Chait, J. Kuriyan & J.E. Darnell "DNA binding of in vitro activated Stat1(, Stat1( and truncated Stat1 interaction between NH2-terminal domains stabilizes binding of two dimers to tandem DNA sites." EMBO J. 15 (1996) 5616 - 5626.

重组STAT 1的C-末端片段的表征 通过MALDI-MS测定烷基化程度， 以及蛋白质的磷酸化。 继续这个项目 将包括测定氨基酸残基， 使用光交联和MALDI-MS肽对DNA结合重要 映射. 一篇描述这项工作的论文已经发表在U。 Vinkemeier，S.L.科恩 I. Moarefi，B.T. Chait，J. Kuriyan & J.E. 体外活化的Stat 1（，Stat 1（和 NH 2-末端结构域之间截短的Stat 1相互作用稳定 两个二聚体与串联DNA位点的结合。“欧洲分子生物学组织J.15（1996）5616 - 5626.