COMPLEX DRIVEN GLOBAL REGULATION OF TRANSCRIPTION

复杂驱动的全球转录监管

• 批准号: 6386505
• 负责人: PHILIP MATSUMURA
• 金额: $ 30.79万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6386505
• 关键词: DNA binding protein; X ray crystallography; bacterial genetics; chemical binding; flagellum; genetic transcription; operon; protein structure function

DESCRIPTION (Adapted from investigator's abstract): The flagellar regulon presents a unique opportunity to study interoperon and global transcriptional regulation. Within the 14 operons in this regulon, there exists a variety of novel mechanisms that ensure the coordination of temporal expression and of transcription with the assembly of the flagellar structure itself. Additionally, it has been shown that one of the gene products of the "master" regulatory operon, FlhD, is a global regulator. This proposal will study how the FlhD/C heterotetramer regulates the flagellar regulon and how FlhD without FlhC regulates other operons. FlhD/C heterotetramer has a different DNA binding specificity than does FlhD by itself. The investigator will determine how the changes in the composition of these complexes alter DNA binding specificity. He will continue comprehensive structure/function analyses of FlhD and complexes containing FlhD. He will use allele-specific suppression analysis and X-ray crystallography to determine how FlhD interacts with FlhC and other possible complex partners. He will also characterize other nonflagellar DNA targets of FlhD and determine how the FlhD- containing complexes interact with these targets.

描述（根据研究者的摘要进行了改编）：鞭毛法规为研究互动和全球转录调节提供了独特的机会。在该调节子的14个操纵子中，存在各种新型机制，可确保时间表的协调和与鞭毛结构本身的组装的转录协调。此外，已经表明，“主”调节操纵子FLHD的基因产物之一是全球监管机构。该提案将研究FLHD/C异驱动器如何调节鞭毛调节器，以及没有FLHC的FLHD如何调节其他操纵子。 FLHD/C异驱动器具有不同于FLHD的DNA结合特异性。研究者将确定这些复合物组成的变化如何改变DNA结合特异性。他将继续对FLHD的全面结构/功能分析和含有FLHD的复合物。他将使用等位基因特异性抑制分析和X射线晶体学来确定FLHD如何与FLHC和其他可能的复杂伴侣相互作用。他还将表征FLHD的其他非Flagellar DNA靶标，并确定含有FLHD的配合物如何与这些靶标相互作用。