TISSUE ENGINEERING OF GROWING JOINTS

关节生长的组织工程

• 批准号: 6171706
• 负责人: JULIANNE GLOWACKI
• 金额: $ 30.46万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6171706
• 关键词: age difference; animal tissue; cell transplantation; chick embryo; connective tissue development; histology; joint prosthesis; joints; laboratory mouse; medical rehabilitation related tag; musculoskeletal transplantation; swine; tissue /cell culture; tissue engineering; tissue support frame; xenotransplantation

The central hypothesis of this project is that a developing joint or skeletal anlage from one species may serve as a morphogenic scaffold for chondrocytes from another species. The anlage will be rendered devoid of innate viable chondrocytes and seeded with chondrocytes from another organism for culture. The source of the viable chondrocytes and thus the cell surface antigens within this engineered growing joint or anlage could therefore be selected. Transplanatation of the engineered construct back into the organism from which the viable chondrocytes had been obtained would be useful in orthopedic and maxillofacial reconstructive surgery. It is our belief that the information obtained from the proposed studies will be translated for clinical application within a short period of time. Our hypothesis will be tested with three major Specific Aims (SA): SA I: The effect of developmental age and thus matrix composition of developing joint scaffolds on the permeation of xenogeneic chondrocytes throughout them will be characterized. SA II: The in vitro growth of engineered joints will be measured. SA III: The fate of in vitro engineered joints in surgical models will be assessed. These studies will first use bovine chondrocytes/chick joint scaffolds because of their low cost. Once technical parameters are defined, mouse chondrocytes/rat joint scaffolds and pig chondrocytes/pig joint scaffolds will be used in preparation for in vivo models. Methods for the in vitro studies include histomorphometric, biochemical and molecular analysis of chondrocyte function. In vivo fate of engineered joints will be assessed in our model of murine joint transplantation and will be further verified in the pig.

该项目的中心假设是，一个发展中的联合或 来自一个物种的骨骼原基可以作为一种形态发生支架， 另一种软骨细胞 原基会被破坏 的软骨细胞，并与来自另一个 有机体培养。 活软骨细胞的来源， 这种工程化生长的关节或原基内的细胞表面抗原 因此可以选择。 移植工程 构建回到生物体中，活的软骨细胞已经 将获得有用的骨科和颌面 重建手术 我们相信，从你的档案中 将翻译用于临床应用 在很短的时间内 我们的假设将用三个 主要特定目标（SA）：SA I：发育年龄的影响， 开发关节支架的基质组合物对 将表征贯穿它们的异种软骨细胞。SA II： 将测量工程化关节的体外生长。SA III：The 将评估手术模型中体外工程化关节的命运。 这些研究将首先使用牛软骨细胞/鸡关节支架 因为它们的成本很低。 一旦确定了技术参数，鼠标 软骨细胞/大鼠关节支架和猪软骨细胞/猪关节 支架将用于制备体内模型。 方法 体外研究包括组织形态学、生物化学和 软骨细胞功能的分子分析。 基因工程的体内命运 将在我们的鼠关节移植模型中评估关节， 将在猪身上进一步验证。