ABNORMAL WATER METABOL IN TYPE 1A ANGIOTENSIN RECEPTOR DEFICIENT MICE: MUTANT

本项目的目标是相关的磁共振和光学组织学 对AET所致脑损伤模型的研究。最近的结果显示 脑组织标本中核磁共振检测到神经元损伤 用DOM处理的动物。磁共振成像中看到的一些病变 标本在常规光学切片上并不明显。 还有尼塞尔·污点。如果这些结果可以在其他模型中得到验证 对于疾病，其影响是巨大的。简而言之，这意味着 这种MRM可以检测出传统组织学中没有看到的病变。这 与中心最近的其他证据一致。共有9个 样本将被扫描。这些是大鼠大脑的1/2 接受过AET治疗。另一半的大脑已经 常规组织学切片分析。其中六个 样本在四个不同的核中显示出显著的损伤：SUBERVER 橄榄核、斜方核、红核、面核。损毁范围 从10%的细胞核到97%的细胞核。我们会 用两种方案扫描第二个1/2大脑：I FSE-TR=1.5秒；TE=50 MS=8；fovz=fovy=10 mm；fovx=20 mm；矩阵(z，y，x)=256×256×128； NEX=2(总时间6.8小时)II GRASS TR=100毫秒TE=3毫秒，阿尔法=350， 与I相同的FOV矩阵，nex=4(总时间=3.64小时)我们将对 MR后的标本，并与特定感兴趣区域进行比较 定量形态计量学描述细胞损伤的百分比。

The goal of this project is correlative MR and optical histology of a model of brain damage induced by AET. Recent results have demonstrated neuronal damage detected by MRM in brain specimens from animals treated with DOM. Some of the lesions seen in the MRM specimens were not evident in conventional optical sections with H&E and Nissl stain. If these results can be demonstrated in other models of disease, the implications are enormous. Simply put, this suggests that MRM can detect lesions not seen in conventional histology. This is consistent with other recent evidence in the Center. A total of 9 specimens will be scanned. These are 1/2 the brains of rats that have been treated with AET. The other half brains have already been sectioned and analyzed with conventional histology. Six of these specimens show significant damage in four different nuclei: Superior Olive, trapezial nucleus, red nucleus, facial nucleus. Damage ranges from 10% of the nucleus to as much as 97% of the nucleus. We will scan the second 1/2 brain with 2 protocols: I FSE -TR=1.5sec; TE=50 ms; Nechos=8; fovz=fovy=10 mm; fovx=20 mm; matrix (z,y,x)=256x256x128; nex=2 (Total time 6.8 hrs) II GRASS TR=100 ms.TE=3 ms, alpha=350 , same fov matrix as I, nex=4 (total time =3.64 hrs) We will section the specimens after MR and compare specific regions of interest with quantitative morphometry to describe % cell damage.