ESTIMATION OF TWO PHOTON DOSE VOLUME BY NUMERICAL SIMULATION

我们的大多数基于MPM的研究涉及深入到 活着的厚厚的样本，从孤立的神经节到活的例子 老鼠。我们还需要一个系统，在这个系统中，笼中试剂和 神经递质激活和电生理学可以进行 同时在成像过程中。需要立式显微镜才能 此系统允许使用长距离的工作距离、水路 沉浸式光学。一种基于奥林巴斯AX-70固定平台的系统 立式显微镜正在建造中，将促进电压 和电流钳位、膜片钳位、连续数据(图) 采集到硬盘上，以及多光子去功能化设施。 还包括用于体内的生理监测仪器 用哺乳动物做实验。这个系统正在我们的许多公司中使用 当前的协作需要在生命系统中进行深度成像。

The majority of our MPM based research involves imaging deep in living thick samples, examples ranging from isolated ganglia to living mice. We also need a system in which both caged reagent and neurotransmitter activation and electrophysiology can be carried out simultaneously during imaging. An upright microscope is required for this system to permit the use of the long working distance, water immersion optics. A system based on a fixed-stage Olympus AX-70 upright microscope is being constructed, and will facilitate voltage and current clamping, patch clamping, continuous data (image) acquisition to hard disk, and facilities for multiphoton uncaging. Also included is physiological monitoring instrumentation for in vivo experiments with mammals. This system is being used in many of our current collaborations that require deep imaging in living systems.