TIME RESOLVED FLUORESCENCE SPECT OF RED SHIFTED GREEN FLUORESCENT PROTEIN MUTANT

红移绿荧光蛋白突变体的时间分辨荧光光谱

• 批准号: 6349436
• 负责人: SAMUEL T HESS
• 金额: $ 0.39万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349436
• 关键词: bioengineering /biomedical engineering; biomedical resource; lasers; plants

Mycorrhizal fungi form mutualistic symbiotic associations with plant roots and are of primary importance in nearly all terrestrial ecosystems. However, very little is known about the cytology of this association, more precisely the cytology of the fungus. AMF produce long hyphae which are not septate (they have no walls between cells in the hyphae). AMF are also multinucleate (they have 2000-5000 nuclei per spore) and when the AMF spores germinate (that is, produce new hyphae) the nuclei concentrated in them wander along the hyphe. It is thought that the "wandering" nuclei are essential to the establishment of the symbiosis (involved in plant-fungus recognition processes), however nothing is known about their behaviour and life cycle before and after meeting the host plant. Techniques have been developed that allow for the establishment of Mycorrhizal plants in tissue culture. The mycorrhizae fungal hyphae are stained with DAPI while growing in agar based growth medium. Using multi photon microscopy we have been able to visualize the plant root-fungi contact areas and study the movement of DAPI-labeled nuclei in fungal hyphae.

菌根真菌形成互惠共生协会， 在几乎所有的陆生植物中， 生态系统 然而，对这种细胞学的了解很少。 关联，更准确地说是真菌的细胞学。 AMF生产 不具隔膜的长菌丝（它们在细胞之间没有壁， 菌丝）。 AMF也是多核的（它们具有2000-5000个核 当AMF孢子萌发时（即产生新的 菌丝）核集中在它们中，沿着菌丝移动。 是 我认为，“游荡”的核是建立 共生（参与植物-真菌识别过程）， 然而，人们对它们的行为和生命周期一无所知， 在与寄主植物相遇后 已经开发出的技术 允许在组织培养中建立菌根植物。 真菌菌丝生长时用DAPI染色， 琼脂基生长培养基。 利用多光子显微镜， 能够可视化植物根真菌接触面积，并研究 真菌菌丝中DAPI标记的细胞核的运动。