MEASURING CONDITIONS FOR HIGH NUMERICAL APERTURE FLUORESCENCE CORRELATION SPECT

我们已经开始开发一种新的快速高效的原型 专为MPM使用而设计的终身成像方案。这 新技术通过以下方式对2PE荧光信号进行电子加权 荧光脉冲的到达时间相对于 通过与线性斜坡相乘的激励脉冲。早到 光子的权重按比例小于延迟到达的光子 反映荧光团的寿命。坡道的作用是提供 模拟信号的时间信息，这比离散时间信息更可取 当收集到数量较少的荧光光子时进行时间入库。 处理电子设备产生成比例的输出 到荧光团的寿命。此技术类似于 快速时间门控方法，但灵敏度潜在较大 较大的低水平荧光，其中统计 光子到达时的波动需要更窄的入库。 此外，集成电子学执行必要的p 煎饼很便宜，而且很容易买到。

We have begun prototype development of a new fast and efficient lifetime imaging scheme specifically designed for use with MPM. This novel technique electronically weights the 2PE fluorescent signal by the time of arrival of the fluorescent pulse relative to the excitation pulse by multiplication with a linear ramp. Early arriving photons are weighted proportionally less than late arriving photons to reflect the lifetime of the fluorophore. The ramp acts to provide analog time information to the signal, which is preferable to discrete time binning when low numbers of fluorescent photons are collected. The processing electronics produce an output that will be proportional to the fluorophore lifetime. This technique is similar to that of the fast time gating method, but the sensitivity is potentially much greater for low levels of fluorescence where the statistical fluctuation in the photon arrivals demands narrower binning. Furthermore, integrated electronics to perform the necessary p rocessing are inexpensive and readily available.