CONCENTRATION MEASUREMENTS ON GFP MUTANTS BY FCS

通过 FCS 测量 GFP 突变体的浓度

• 批准号: 6349409
• 负责人: SAMUEL T HESS
• 金额: $ 0.22万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349409
• 关键词: bioengineering /biomedical engineering; biomedical resource; human tissue; lipids; technology /technique

Work has continued on the effort to measure the fusion of single vesicles to planar membranes. Systems have been developed for the formation of large unilamellar vesicles and planar membranes, and we are working towards reliably patch clamping them. A novel electrical configuration has been adopted in which the pipette and surrounding bath are maintained at ground potential, and the solution on the trans side of the bilayer is stimulated with an a.c. voltage. This configuration sidesteps the normal problems associated with large pipette and membrane capacitances and provides for a nearly background free measurement limited only by amplifier noise.

测量单细胞融合的工作仍在继续 囊泡到平面膜。已经开发出了用于 大的单层囊泡和平面膜的形成，我们 正在致力于可靠地膜片钳住它们。一种新型电器 采用了吸管和周围环境的配置 镀液保持在地电位，而溶液在传输 双层的一侧用交流电刺激。电压。这 配置避开了与 移液管和膜电容，并提供近乎背景 自由测量仅受放大器噪声的限制。