CONCENTRATION MEASUREMENTS ON GFP MUTANTS BY FCS

通过 FCS 测量 GFP 突变体的浓度

• 批准号: 6349409
• 负责人: SAMUEL T HESS
• 金额: $ 0.22万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349409
• 关键词: bioengineering /biomedical engineering; biomedical resource; human tissue; lipids; technology /technique

Work has continued on the effort to measure the fusion of single vesicles to planar membranes. Systems have been developed for the formation of large unilamellar vesicles and planar membranes, and we are working towards reliably patch clamping them. A novel electrical configuration has been adopted in which the pipette and surrounding bath are maintained at ground potential, and the solution on the trans side of the bilayer is stimulated with an a.c. voltage. This configuration sidesteps the normal problems associated with large pipette and membrane capacitances and provides for a nearly background free measurement limited only by amplifier noise.

工作一直在努力衡量单一的融合 囊泡到平面膜。 已经开发了系统的系统 大型单层囊泡和平面膜的形成，我们 正在努力可靠地夹紧它们。 一种新颖的电气 采用了移液管和周围的配置 沐浴保持地面电势，并在反式上进行溶液 双层的侧面被A.C刺激电压。 这 配置避开了与大型相关的正常问题 移液器和膜电容，并提供几乎背景 免费测量仅受放大器噪声限制。