课题基金 / 基金详情

IN VIVO MULTIPHOTON FLUORESCENCE PHOTOBLEACHING RECOVERY (MPFPR) MEASUREMENTS

IN VIVO MULTIPHOTON FLUORESCENCE PHOTOBLEACHING RECOVERY (MPFPR) MEASUREMENTS
体内多光子荧光光漂白恢复 (MPFPR) 测量
批准号:
6349385
负责人:
WARREN R ZIPFEL
金额:
$0.25万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-09-01 至 2001-08-31

项目摘要

项目成果

WARREN R ZIPFEL的其他基金

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中文摘要
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英文摘要
The major drawback of pulsed two-photon excitation schemes in fluorescence correlation spectroscopy compared to one-photon applications is the existence of comparably long dark periods (12ns) for pulsed excitation, since the fluorescence lifetime is for most fluorophors only several ns. By splitting the laser beam and using different delay lines, we built a pulse doubler and quadrupler that was applied to FCS. The signal-to-noise ratio could be improved by a factor of 1.5 with proper alignment.
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Femto-seq: Targeted photo-biotinylation, pulldown and sequencing of locus and region-specific DNA from femtoliter volumes within individual cells
  • 批准号:
    10587721
  • 项目类别:
  • 资助金额:
    $42.13万
  • 财政年份:
    2023
  • 负责人:
    WARREN R ZIPFEL
  • 依托单位:
Broad wavelength range Zeiss 780 NLO/confocal system for the Cornell Imaging Core
  • 批准号:
    8734809
  • 项目类别:
  • 资助金额:
    $83.44万
  • 财政年份:
    2014
  • 负责人:
    WARREN R ZIPFEL
  • 依托单位:
A miniature confocal for long term 3D multicolor imaging within a C02 incubator
  • 批准号:
    8575684
  • 项目类别:
  • 资助金额:
    $16.82万
  • 财政年份:
    2013
  • 负责人:
    WARREN R ZIPFEL
  • 依托单位:
CONFOCAL MICROSCOPE: GENES & GENOME
  • 批准号:
    7166427
  • 项目类别:
  • 资助金额:
    $12.5万
  • 财政年份:
    2005
  • 负责人:
    WARREN R ZIPFEL
  • 依托单位: