Multiphoton Detection of Cancer

癌症的多光子检测

• 批准号: 7454443
• 负责人: WARREN R ZIPFEL
• 金额: $ 39.65万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7454443
• 关键词: Air; Animal Cancer Model; Animals; Anisotropy; Area; Arts; Cancer Biology; Cancer Detection; Cancer Model; Cells; Clinical; Collagen; Collection; Coupled; Depth; Detection; Development; Diagnosis; Diagnostic; Dysplasia; Effectiveness; Electronics; Endoscopes; Endoscopy; Engineering; Evaluation; Event; Exhibits; Eye; Facility Construction Funding Category; Fiber; Financial compensation; Fluorescence; Funding; Generations; Goals; Histological Techniques; Human; Image; Implant; Investigation; Laboratories; Lasers; Leadership; Life; Malignant - descriptor; Malignant Neoplasms; Measurement; Medical; Methods; Microscope; Microscopic; Microscopy; Morphologic artifacts; Morphology; Motion; Neoplasm Metastasis; Optics; Ovarian; Physics; Physiologic pulse; Property; Prostatic Neoplasms; Pulse taking; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Scanning; Scheme; Science; Screening procedure; Signal Transduction; Silicon Dioxide; Solid; Specimen; Spectrum Analysis; Staging; Standards of Weights and Measures; Technology; Time; Tissues; Translations; Universities; Use Effectiveness; adaptive optics; analog; angiogenesis; base; carcinogenesis; clinical application; design; detector; fluorescence imaging; fluorophore; in vivo; instrument; interdisciplinary approach; mouse model; multidisciplinary; novel; novel diagnostics; photonics; programs; prototype; research study; second harmonic; spectroscopic imaging; tool; tumor progression; two-photon

DESCRIPTION (provided by applicant): The goal of this project is to characterize and implement the unique properties of multiphoton excited fluorescence and nonlinear scattering for in vivo detection of cancer and novel in vivo studies in cancer biology. An engineering goal of this proposal is to construct a fully functional laser-scanning multiphoton endoscope capable of producing images of living tissue using primarily intrinsic fluorophores and collagen second harmonic signals that are of the resolution and quality currently obtainable; with a state-of-the-art multiphoton microscope. To accommodate the breath of this multidisciplinary proposal, there are three laboratories and a biomedical instrument company (Optiscan Imaging Ltd) involved, whose combined expertise encompasses all of the science and technologies required to successfully complete the project. The project leadership is centered at the NIH/NIBIB Developmental Resource for Biophysical Imaging and Opto-electronics (DRBIO) at Cornell University in the department of Applied and Engineering Physics. The three specific aims are: 1) A direct comparison of multiphoton imaging and spectroscopic parameters of intrinsic tissue signals with standard histological techniques, focusing on the discovery of new diagnostic criteria for cancer. 2) Construction of a raster-scanning fiber endoscope for multiphoton emission imaging in tissue. 3) Optimization of multiphoton microscopy for imaging mouse models of cancer, and an exploration of the potential of large field-of-view (~ 1cm2) multiphoton imaging as a diagnostic tool for clinical use. The overall goal of this program is to develop a new and powerful infrastructure for noninvasive, microscopic medical detection and diagnosis of premalignancy and cancer. We anticipate that methods established will be of general value for characterizing of carcinogenesis in other cancer models, and ultimately in human clinical applications.

项目描述（由申请人提供）：本项目的目标是描述和实现多光子激发荧光和非线性散射的独特特性，用于癌症的体内检测和癌症生物学的新体内研究。本提案的工程目标是构建一个功能齐全的激光扫描多光子内窥镜，能够使用当前可获得的分辨率和质量的主要固有荧光团和胶原蛋白二次谐波信号产生活组织图像；用最先进的多光子显微镜。