Zeiss 510 Multiphoton/single-photon Confocal Microscope

Zeiss 510 多光子/单光子共焦显微镜

• 批准号: 6877387
• 负责人: WARREN R ZIPFEL
• 金额: $ 50万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877387
• 关键词: biological signal transduction; biomedical equipment purchase; confocal scanning microscopy; molecular /cellular imaging

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a Zeiss 510 multiphoton/single-photon confocal microscope to be housed in the Department of Biomedical Sciences in the Cornell College of Veterinary Medicine. The goal is to provide a modern, user-friendly fluorescence imaging system for use by PHS-funded researchers located in Biomedical Sciences and other departments within the Veterinary College. The microscope will also become part of the newly established Cornell Imaging Core, a campus-wide initiative to provide state-of-art imaging for all biological and biomedical researchers at the Ithaca campus. As part of the core, the instrument will be made available to PHS-funded researchers from other departments on the Cornell campus as scheduling permits. This equipment is required due to an increase in vivo imaging projects at Cornell involving rodent and transgenic mouse models of disease and pathophysiology. In addition, several other PHS-funded projects that require microscopic imaging of cell cultures will also benefit from the multicolor (single photon) confocal imaging capabilities of the Zeiss 510. Researchers from Biomedical Sciences whose projects involve in vivo imaging are currently using the multiphoton imaging systems of Developmental Resource for Biophysical Imaging and Opto-electronics (DRBIO), which is located in the School of Applied and Engineering Physics at Cornell. DRBIO is funded by NIBIB via the P41 mechanism and is a national Resource, and therefore cannot give priority to Cornell researchers over PHS-funded users from other universities. Recently time on DRBIO's three multiphoton microscopes suitable for in vivo imaging projects has become limited due to high demand. For example, DRBIO has collaborators scheduling time as much as three months in advance. In addition, infrastructure demands of live animal imaging, such as adequate ventilation of anesthesia gases and proximity to specialized facilities for animal surgery have become more difficult to meet due to building constraints and new Environmental Health and Safety requirements. Finally, transportation of transgenic animals between DRBIO and the transgenic mouse facility, located a mile away, creates a constant aggravation. Infrastructure and transportation problems will be completely alleviated by placement of a multiphoton imaging system near the transgenic mouse facility in the Veterinary College.

描述(由申请人提供)：该建议书申请资金购买蔡司510多光子/单光子共焦显微镜，安装在康奈尔兽医学院生物医学科学系。其目标是提供一种现代化的、用户友好的荧光成像系统，供位于生物医学科学和兽医学院其他系的PHS资助的研究人员使用。该显微镜还将成为新成立的康奈尔成像核心的一部分，这是一项全校范围的倡议，旨在为伊萨卡校园的所有生物和生物医学研究人员提供最先进的成像。作为核心的一部分，该仪器将提供给康奈尔大学校园内其他部门的PHS资助的研究人员作为日程安排许可。由于康奈尔大学增加了涉及啮齿动物和转基因小鼠疾病和病理生理学模型的活体成像项目，因此需要这种设备。此外，小灵通资助的其他几个需要细胞培养显微成像的项目也将受益于蔡司510的多色(单光子)共焦成像能力。来自生物医学科学的研究人员的项目涉及活体成像，目前正在使用生物物理成像和光电子学开发资源(DRBIO)的多光子成像系统，该系统位于康奈尔大学应用和工程物理学院。DRBIO是由NIBIB通过P41机制资助的，是国家资源，因此不能优先考虑康奈尔大学的研究人员，而不是其他大学由PHS资助的用户。最近，由于需求很高，DRBIO的三台多光子显微镜适用于活体成像项目的时间变得有限。例如，DRBIO让协作者提前三个月安排时间。此外，由于建筑限制和新的环境健康和安全要求，活体动物成像的基础设施需求，如麻醉气体的充分通风和靠近专门的动物手术设施，已变得更加难以满足。最后，在DRBIO和位于一英里之外的转基因小鼠设施之间运送转基因动物，造成了持续不断的恶化。通过在兽医学院转基因小鼠设施附近放置多光子成像系统，基础设施和交通问题将完全缓解。