Broad wavelength range Zeiss 780 NLO/confocal system for the Cornell Imaging Core
适用于康奈尔成像核心的宽波长范围 Zeiss 780 NLO/共焦系统
基本信息
- 批准号:8734809
- 负责人:
- 金额:$ 83.44万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-07-15 至 2015-07-14
- 项目状态:已结题
- 来源:
- 关键词:16 year oldArtsBiotechnologyCollaborationsComputer softwareCore FacilityCoupledDetectionDevelopmentFamilyFluorescenceFluorescent DyesFundingFutureGoalsHeadHousingImageLasersLifeMicroscopeMicroscopyMinorOperating SystemPhotobleachingPhotonsPhysicsProteinsRequest for ProposalsResearch PersonnelResourcesRunningSapphireSourceSystemTimeUnited States National Institutes of HealthUniversitiesanimal tissuedetectorfluorescence imaginginsightinstrumentphotoactivationquantumtissue preparationtwo-photonuser-friendly
项目摘要
DESCRIPTION (provided by applicant): This high-end S10 proposal requests funds to purchase a Zeiss LSM780 NLO multiphoton/single-photon confocal microscope coupled to a broad tuning femtosecond laser from Newport/Spectra-Physics (the Insight laser). The instrument will be housed in the Cornell Biotechnology Resource Center's (BRC) Imaging Core facility in the basement of Weill Hall on the Ithaca campus of Cornell University. The goal is to provide a modern, user-friendly combined nonlinear and confocal fluorescence imaging system for use by ten NIH-funded major users and 6 minor users located on the Cornell campus. The instrument will replace an outdated Bio-Rad 1024 multiphoton system which was installed originally in 1996 in the P41 Developmental Resource (DRBIO) in collaboration with Bio-Rad, the initial licensee of multiphoton microscopy. The instrument was later taken over by the Cornell Biotechnology Resource Center (BRC) imaging facility which serves the entire campus. It has been retrofitted numerous times, but still runs under the defunct IBM OS/2 operating system and the Bio-Rad LaserSharp software package rendering it completely un-supportable. Acquisition of the state-of-art system requested will greatly advance the capabilities of the Cornell Imaging facility, and allow us to better serve the needs of our NIH funded researchers. This instrument will not only be replacing an unsupportable 16 year old multiphoton microscope, but will be adding several new previously unavailable features that will be of great benefit to our
users. These include two-photon excitation wavelengths up to 1300 nm that will provide for deeper imaging with higher viability, and, for the first time, allow our users to assess families o red dyes and fluorescent proteins that are currently inaccessible with our standard Ti:Sapphire source. The system also has the most up-to-date highest quantum efficiency detectors - both in the confocal head and the external non-descanned detector system. All of the users of this proposed microscope will be imaging live animals, tissues or tissue preparations and all will benefit from the 2 to 3 fold increase in fluorescence detection these newer photodetectors provide. Equally important, the Zeiss system and Zen software provide extremely precise control of the laser excitation as required by our users who have a need for 3D localized photoactivation, photobleaching and targeted photoablation. The requested instrument is specifically tailored to the needs of our NIH funded major and minor users, and will provide for their fluorescence imaging needs well into the future.
描述(由申请人提供):该高端S10提案要求提供资金,用于购买蔡司LSM 780 NLO多光子/单光子共焦显微镜,该显微镜与来自纽波特/光谱物理公司的宽调谐飞秒激光器(Insight激光器)耦合。该仪器将被安置在康奈尔生物技术资源中心(BRC)的成像核心设施,位于康奈尔大学伊萨卡校区威尔大厅的地下室。我们的目标是提供一个现代化的,用户友好的组合非线性和共聚焦荧光成像系统,供10个NIH资助的主要用户和6个位于康奈尔大学校园的次要用户使用。该仪器将取代过时的Bio-Rad 1024多光子系统,该系统最初于1996年安装在P41开发资源(DRBIO)中,与Bio-Rad合作,Bio-Rad是多光子显微镜的最初许可证。该仪器后来被康奈尔大学生物技术资源中心(BRC)的成像设施,服务于整个校园。它已经被改造了无数次,但仍然运行在已不存在的IBM OS/2操作系统和Bio-Rad LaserSharp软件包下,使其完全不受支持。获得所要求的最先进的系统将大大提高康奈尔成像设施的能力,并使我们能够更好地满足我们NIH资助的研究人员的需求。这台仪器不仅将取代一个不支持的16岁的多光子显微镜,但将增加几个新的以前不可用的功能,这将是非常有益的,
用户.其中包括高达1300 nm的双光子激发波长,这将提供更深的成像和更高的可行性,并首次允许我们的用户评估目前无法使用我们的标准Ti:Sapphire源的红色染料和荧光蛋白家族。该系统还具有最新的最高量子效率探测器-共焦头和外部非去扫描探测器系统。所有的用户提出的这种显微镜将成像活动物,组织或组织制剂,所有将受益于2至3倍增加荧光检测这些新的光电探测器提供。同样重要的是,Zeiss系统和Zen软件可根据需要进行3D局部光活化、光漂白和靶向光消融的用户的要求,提供极其精确的激光激发控制。所要求的仪器是专门为我们的NIH资助的主要和次要用户的需求量身定制的,并将在未来很长一段时间内满足他们的荧光成像需求。
项目成果
期刊论文数量(42)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Metallo-β-lactamase domain-containing protein 2 is S-palmitoylated and exhibits acyl-CoA hydrolase activity.
- DOI:10.1074/jbc.ra120.015701
- 发表时间:2021-01
- 期刊:
- 影响因子:0
- 作者:Malgapo MIP;Safadi JM;Linder ME
- 通讯作者:Linder ME
Photoactivated in Vitro Anticancer Activity of Rhenium(I) Tricarbonyl Complexes Bearing Water-Soluble Phosphines.
- DOI:10.1021/acs.inorgchem.7b02747
- 发表时间:2018-02-05
- 期刊:
- 影响因子:4.6
- 作者:Marker SC;MacMillan SN;Zipfel WR;Li Z;Ford PC;Wilson JJ
- 通讯作者:Wilson JJ
Dietary sphinganine is selectively assimilated by members of the mammalian gut microbiome.
- DOI:10.1194/jlr.ra120000950
- 发表时间:2021
- 期刊:
- 影响因子:6.5
- 作者:Lee MT;Le HH;Johnson EL
- 通讯作者:Johnson EL
A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex.
AP2 复合物磷酸化依赖性失活的结构机制。
- DOI:10.7554/elife.50003
- 发表时间:2019
- 期刊:
- 影响因子:7.7
- 作者:Partlow,EdwardA;Baker,RichardW;Beacham,GwendolynM;Chappie,JoshuaS;Leschziner,AndresE;Hollopeter,Gunther
- 通讯作者:Hollopeter,Gunther
Substrate stiffness heterogeneities disrupt endothelial barrier integrity in a micropillar model of heterogeneous vascular stiffening.
在异质血管硬化的微柱模型中,基质硬度异质性破坏了内皮屏障的完整性。
- DOI:10.1039/c8ib00124c
- 发表时间:2018
- 期刊:
- 影响因子:0
- 作者:VanderBurgh,JacobA;Hotchkiss,Halie;Potharazu,Archit;Taufalele,PaulV;Reinhart-King,CynthiaA
- 通讯作者:Reinhart-King,CynthiaA
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WARREN R ZIPFEL其他文献
WARREN R ZIPFEL的其他文献
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