EFFECTS OF CADMIUM AND MERCURY ON NA-K-CL COTRANSPORTER IN SHARK RECTAL GLAND

镉和汞对肾脏有极强的毒性，对 它们的作用机制是防止或 逆转它们对肾脏的有害影响。这项研究是设计的 以深入了解重金属对严重环境的影响 参与尿液浓缩过程。用作隔离的模型 鲨鱼直肠腺的质膜，是一种丰富的 他们的作用机制将是第一个 关于离子转运和布美塔尼的动力学特征 结合部位。然后参与这一过程的氨基酸侧链 重金属与特定试剂之间的相互作用 毒素。最后，携带这些重金属的氨基酸- 敏感侧链将在分子水平上定域。 这些研究将大大有助于理解 重金属在转运蛋白水平上的作用 提供有关潜在目标地点、假定地点的重要信息 重金属及其化学物质的受体和可能的结合基序 大自然。

Cadmium and mercury are extremely nephrotoxic and the understanding of their mechanism of action is one essential step in preventing or reversing their detrimental effect on the kidney. This study is designed to provide insights into the action of heavy metals on critically involved in the urinary concentrating process. Using as a model isolated plasma membranes of the rectal gland of the shark, which are an abundant source for this cotransporter, their mechanism of action will be first characterized kinetically with regard tot he ion transport and bumetanide binding sites. Then the amino acid side chains involved in this interaction between heavy metals and the specific reagents with that of the toxins. Finally, the amino acids carrying these heavy metal- sensitive side chains will be localized at the molecular level. These studies will contribute substantially to the understanding of the action of heavy metals at the transporter protein level and thereby provide important information on potential target sites, putative receptors and possible binding motifs of heavy metals and their chemical nature.