EFFECTS OF CADMIUM AND MERCURY ON NA-K-CL COTRANSPORTER IN SHARK RECTAL GLAND

镉和汞对鲨鱼直肠腺NA-K-CL协同转运蛋白的影响

• 批准号: 6575670
• 负责人: ROLF KINNE
• 金额: $ 17.41万
• 依托单位: MOUNT DESERT ISLAND BIOLOGICAL LAB
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6575670
• 关键词: bumetanide; cadmium; cell membrane; chlorine; immunoprecipitation; ion transport; membrane transport proteins; mercury; metal poisoning; potassium; proteolysis; renal toxin; rubidium; salt gland; saltwater environment; sharks; sodium; tissue /cell culture; toxicant interaction

Cadmium and mercury are extremely nephrotoxic and the understanding of their mechanism of action is one essential step in preventing or reversing their detrimental effect on the kidney. This study is designed to provide insights into the action of heavy metals on critically involved in the urinary concentrating process. Using as a model isolated plasma membranes of the rectal gland of the shark, which are an abundant source for this cotransporter, their mechanism of action will be first characterized kinetically with regard tot he ion transport and bumetanide binding sites. Then the amino acid side chains involved in this interaction between heavy metals and the specific reagents with that of the toxins. Finally, the amino acids carrying these heavy metal- sensitive side chains will be localized at the molecular level. These studies will contribute substantially to the understanding of the action of heavy metals at the transporter protein level and thereby provide important information on potential target sites, putative receptors and possible binding motifs of heavy metals and their chemical nature.

镉和汞具有极强的肾毒性， 其作用机制是预防或 逆转它们对肾脏的有害影响。 本研究设计 提供深入了解重金属对关键 参与尿液浓缩过程。 作为一个模型， 鲨鱼直肠腺的质膜，这是一个丰富的 这种协同转运蛋白的来源，它们的作用机制将首先 离子转运和布美他尼的动力学特征 结合位点。 那么与此相关的氨基酸侧链 重金属与特定试剂的相互作用 毒素 最后，携带这些重金属的氨基酸- 敏感侧链将定位在分子水平。 这些研究将大大有助于了解 重金属在转运蛋白水平的作用， 提供关于潜在靶位点的重要信息， 重金属及其化学物质的受体和可能的结合基序 自然