Functional Genomics of Drosphila Innate Immunity

果蝇先天免疫的功能基因组学

• 批准号: 6405489
• 负责人: BRIAN R BETTENCOURT
• 金额: $ 3.33万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-02 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6405489
• 关键词: Drosophilidae; Pseudomonas; Serratia; arthropod genetics; bactericidal immunity; biochemical evolution; biotechnology; functional /structural genomics; gene expression; genetic polymorphism; genetic regulatory element; genetic strain; microarray technology; polymerase chain reaction; quantitative trait loci

This study aims to conduct a functional genomic analysis of variation in the efficacy of innate immune defense in Drosophila melanogaster, to enhance the understanding of the genetic bases of innate immunity. Insect innate immunity is an excellent model for vertebrate preadaptive immunity. The research conducted in this study will accomplish two key health-related goals: the identification of novel antimicrobial proteins of antibiotic-design potential, and importantly, the identification of host genes responsible for variation among individuals in immune defense as potential therapeutic targets. The specific aims of the study are: First, to quantify the expression kinetics of immune defense genes after infection with a natural pathogen in Drosophila strains that vary in their ability to defend against infection. Second, to apply DNA microarray analysis to these strains, in order to identify novel defense-gene regulatory targets, quantify variation in the kinetics of genome-wide expression during infection, and to identify sequence divergence contributing to known immunity quantitative trait loci.

这项研究旨在对果蝇果蝇的先天免疫防御功效的变异进行功能性基因组分析，以增强对先天免疫遗传基础的理解。昆虫先天免疫是脊椎动物前预防免疫的绝佳模型。这项研究中进行的研究将实现两个与健康相关的关键目标：鉴定抗生素设计潜力的新型抗菌蛋白，重要的是，鉴定了负责免疫防御中的宿主基因作为潜在治疗靶点的宿主基因。该研究的具体目的是：首先，量化果蝇菌株中自然病原体感染后免疫防御基因的表达动力学，它们在抗感染的能力方面有所不同。其次，要将DNA微阵列分析应用于这些菌株，以识别新的防御基因调节靶标，可以量化感染过程中全基因组表达动力学的变化，并确定有助于已知免疫定量性状性状基因座的序列发散。