CORE--ANALYTICAL AND APPLIED NEUROSCIENCE

核心——分析和应用神经科学

• 批准号: 6338913
• 负责人: Richard B Mailman
• 金额: $ 20.78万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6338913
• 关键词: analytical chemistry; analytical method; autoradiography; behavior disorders; biomedical facility; electrospray ionization mass spectrometry; experimental designs; gas chromatography; gene targeting; high performance liquid chromatography; in situ hybridization; mental disorders; method development; neuroendocrine system; neurosciences; pharmacokinetics; psychopharmacology; radioimmunoassay

The primary goal of the Analytical and Applied Neuroscience (AAN) Core is to be the bridge (both technically and theoretically) between clinical hypotheses and the basic neuroscience and analytical parameters required for their testing. Thus, the AAN Core provides MHCRC scientist with quantification of any analyte (endogenous or exonobiotic) that will assist in conducting an approved study or the development of a preclinical research strategy that will address the investigator's hypotheses. Moreover, this Core provides these services in conjunction with consultation about the strengths and limitations of such methods and strategies for the desired studies. The services provided are cost-efficient, and often represent assistance that the investigator either could not readily obtain, or could obtain only with extreme difficulty or expense. The core uses diverse methodologies [ranging from radioimmunoassays and High Performance Liquid Chromatography (HPLC), to HPLC-electrospray mass spectrometry (HPLC-ESMS)] to quantify such molecules that are critical to the testing of scientific hypotheses, both clinical and basic. As noted above, not only does the core implement or develop the necessary techniques and perform the assays, but it also provides consultation to clinical investigators related to study design (e.g., utility of measures, sample acquisition and storage, interpretation, etc.). Finally, with approval of the MHCRC Scientific Review Committee, this core provides analytical service and collaboration to MHCRC-associated basic scientists, and by so doing, solidifies the vital link between clinic and basic scientists and their exchange of ideas. It is the range of the services provided by the core that led to its present name. The core also provides technical assistance and resources to basic MHCRC scientists for procedures and services that their work requires, but for which they have neither sufficient expertise nor means. The key to accomplishing these ends is the ability of this core to provide state-of- the-art analytical assays of both endogenous molecules and xenobiotics (and their metabolites). During the past five years, the MHCRC has taken several steps to maximize the efficiency and responsiveness of achieving these ends. Yet it is imperative to note that the AAN Core is a single unit that provides services that were formerly the domain of three separate laboratory-based cores which had been approved in the last competing application (see below). The background and rationale for this significant change in organization are discussed in detail in Section C.1.

分析和应用神经科学（AAN）的主要目标 核心是成为两者之间的桥梁（技术上和理论上） 临床假设以及基础神经科学和分析 测试所需的参数。 因此，AAN 核心提供 MHCRC 科学家对任何分析物（内源性或 exonobiotic）将有助于进行批准的研究或 制定临床前研究策略，以解决 研究者的假设。 此外，该核心还提供这些服务 结合有关优势和局限性的咨询 所需研究的此类方法和策略。 服务 所提供的援助具有成本效益，并且通常代表 研究者要么无法轻易获得，要么只能通过 极其困难或费用。 核心使用不同的方法 [范围从放射免疫分析和高性能液体 色谱 (HPLC)、HPLC-电喷雾质谱 (HPLC-ESMS)] 来量化对这些至关重要的分子 检验科学假设，包括临床和基础。 如前所述 如上所述，核心不仅实现或开发必要的 技术并进行分析，但它也提供咨询 与研究设计相关的临床研究者（例如，措施的效用， 样本采集和存储、解释等）。 最后，与 MHCRC 科学审查委员会的批准，这一核心 为 MHCRC 相关机构提供分析服务和协作 基础科学家，通过这样做，巩固了临床之间的重要联系 和基础科学家及其思想交流。 它的范围是 由核心提供的服务导致其现在的名称。 核心 还为基本 MHCRC 提供技术援助和资源 科学家的工作所需的程序和服务，但 他们既没有足够的专业知识，也没有足够的手段。 关键是 实现这些目标是该核心提供状态的能力 内源性分子和外源性物质的最先进的分析测定 （及其代谢物）。 在过去的五年里，MHCRC 采取了几个步骤来最大限度地提高效率和响应能力 实现这些目标。 但必须指出的是，AAN 核心 是一个提供以前属于以下领域的服务的单一单位 三个独立的基于实验室的核心已在 最后的竞争申请（见下文）。背景和理由 对于组织中的这一重大变化，我们详细讨论了 C.1 节。